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Artigo Revisado por pares
BIBTEX RIS

Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus

2020; Elsevier BV; Volume: 289; Linguagem: Inglês

10.1016/j.jviromet.2020.114056

ISSN

1879-0984

Autores

Olga V. Orlova, Д. В. Глазкова, Г. М. Цыганова, Irina V. Antoshkina, R. R. Mintaev, Andrey Tikhonov, E. V. Bogoslovskaya, German A. Shipulin,

Tópico(s)

Virus-based gene therapy research

Resumo

Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens.

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