Produção Nacional
Revisado por pares
Interaction of Genetically Encoded Photosensitizers with Scintillating Nanoparticles for X-ray Activated Photodynamic Therapy
2021; American Chemical Society; Volume: 13; Issue: 2 Linguagem: Inglês
10.1021/acsami.0c19041
ISSN1944-8252
AutoresMariana C. Micheletto, Éder José Guidelli, Antonio J. Costa‐Filho,
Tópico(s)bioluminescence and chemiluminescence research
ResumoPhotodynamic therapy (PDT) applications are limited by the low penetration of UV–visible light into biological tissues. Considering X-rays as an alternative to excite photosensitizers (PS) in a deeper tumor, an intermediate particle able to convert the X-ray energy into visible light (scintillating nanoparticle, ScNP) is necessary. Moreover, accumulation of PS in the target cells is also required. Genetically encoded proteins could be used as a photosensitizer, allowing the exclusive expression of PS inside the tumor cells. Here, the interaction of eGFP, KillerOrange, and KillerRed proteins with LaF3:Tb3+ ScNP was investigated, for the first time, in terms of its physicochemical and energy transfer properties. The protein structure, stability, and function were evaluated upon adverse physiological conditions and X-ray irradiation. Optimal parameters for energy transfer from ScNP to the proteins were investigated, paving the way for the use of genetically encoded photosensitizers for applications in X-ray activated photodynamic therapy.
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