Design of an Intergrated Silicon-PDMS Cell Cartridge
1998; Linguagem: Inglês
10.31438/trf.hh1998.83
AutoresB.Derek DeBusschere, David A. Borkholder, G.T.A. Kovacs,
Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoThe use of cell based biosensors for applications outside of the laboratory has been limited in part due to packaging issues.A design for an integrated cell cartridge that addresses the require ments of sterile fluidic interconnect and environmental regulation is presented.The device consists of a PDMS (polydimethylsiloxane) part, a glass cover, and a silicon sensing die mounted on a printed circuit board.The PDMS part forms the fl uidic channels, intercon nect ports, septa, and two IO µl chambers over the active sensing area.The silicon die will include integrated biological sensors and a temperature regulation system, and the glass cover seals the cham bers.Electrical and fluidic connections are made simultaneously as needles pierce septa on the cartridge when it is plugged into a zero insertion force (ZIF) socket.The viability of injecting suspended cells into a 10 µl volume chamber and culturing them for greater than one week using a con tinuous fl ow perfusion system has been demonstrated.Initial proto types of the cell cartridges have been assembled and cells have been cultured in the 10 µl PDMS chambers.
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