Biomimetic Keratin Gold Nanoparticle-Mediated In Vitro Photothermal Therapy on Glioblastoma Multiforme
2021; Future Medicine; Volume: 16; Issue: 2 Linguagem: Inglês
10.2217/nnm-2020-0349
ISSN1748-6963
AutoresAlexa Guglielmelli, Paolo Rosa, Marco Contardi, Mirko Prato, Giorgio Mangino, Selenia Miglietta, Vincenzo Petrozza, R. Pani, Antonella Calogero, Athanassia Athanassiou, Giovanni Perotto, Luciano De Sio,
Tópico(s)Nanoplatforms for cancer theranostics
ResumoAim: To realize and characterize a new generation of keratin-coated gold nanoparticles (Ker-AuNPs) as highly efficient photosensitive nanosized therapeutics for plasmonic photothermal (PPT) therapy. Materials & methods: The chemical, physical, morphological and photothermal properties of Ker-AuNPs are investigated using dynamic light scattering, ζ-potential, UV-Visible, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, transmission electron microscopy and high-resolution thermography. In vitro experiments are performed on a human glioblastoma cell line (i.e., U87-MG), using viability assays, transmission electron microscopy, fluorescence microscopy, cytometric analyses and PPT experiments. Results: Experiments confirm the excellent biocompatibility of Ker-AuNPs, their efficient cellular uptake and localized photothermal heating capabilities. Conclusion: The reported structural and functional properties pointed out these Ker-AuNPs as a promising new tool in the field of biocompatible photothermal agents for PPT treatments against cancer-related diseases.
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