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Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems

2021; American Association for the Advancement of Science; Volume: 371; Issue: 6528 Linguagem: Inglês

10.1126/science.aax2656

1095-9203

Shahar Alon, Daniel Goodwin, Anubhav Sinha, Asmamaw T. Wassie, Fei Chen, Evan R Daugharthy, Yosuke Bando, Atsushi Kajita, Andrew G. Xue, Karl Marrett, Robert Prior, Yi Cui, Andrew C. Payne, Chun‐Chen Yao, Ho‐Jun Suk, Ru Wang, Chih-Chieh Yu, Paul W. Tillberg, Paul Reginato, Nikita Pak, Songlei Liu, Sukanya Punthambaker, Eswar Prasad R. Iyer, Richie E. Kohman, Jeremy A. Miller, Ed S. Lein, Ana Lako, Nicole Cullen, Scott J. Rodig, Karla Helvie, Daniel L. Abravanel, Nikhil Wagle, Bruce E. Johnson, Johanna Klughammer, Michal Slyper, Julia Waldman, Judit Jané‐Valbuena, Orit Rozenblatt–Rosen, Aviv Regev, George M. Church, Adam Marblestone, Edward S. Boyden, H. Raza Ali, M. Al Sa’d, Shahar Alon, Samuel Aparício, Giorgia Battistoni, Shankar Balasubramanian, Robert Becker, Bernd Bodenmiller, Edward S. Boyden, Dario Bressan, Alejandra Bruna, Marcel Burger, Carlos Caldas, Maurizio Callari, Ian G. Cannell, Helen Casbolt, N. Chornay, Yi Cui, A. Dariush, K. Dinh, A. Emenari, Y. Eyal-Lubling, Jean Fan, Ali Fatemi, Edward A. Fisher, E. A. González-Solares, C. Gónzalez-Fernández, Daniel Goodwin, Wendy Greenwood, Francesco Grimaldi, Gregory J. Hannon, Owen Harris, Shelley Harris, Cristina Jauset, Johanna A. Joyce, Emmanouil D. Karagiannis, Tatjana Kovačević, Laura Kuett, Russell Kunes, A. Yoldaş, Dongbing Lai, Emma Laks, Hsuan Lee, M. Lee, Giulia Lerda, Yuxin Li, Andrew McPherson, Neal L. Millar, Claire M. Mulvey, Fiona Nugent, Ciara H. O’Flanagan, Marta Pàez‐Ribes, I. Pearsall, Fatime Qosaj, Andrew Roth, Oscar M. Rueda, Teresa Ruíz, Kirsty Sawicka, Leonardo A. Sepúlveda, Sohrab P. Shah, Abigail Shea, Anubhav Sinha, Adrian L. Smith, S. Tavaré, Sandra Tietscher, Ignacio Vázquez-Garćıa, Siegfried Vogl, N. A. Walton, Asmamaw T. Wassie, Spencer S. Watson, Joanna Weselak, Sonja Wild, Elena Williams, Jonas Windhager, Tristan Whitmarsh, Chuan Xia, Ping Zheng, Xiaowei Zhuang,

CRISPR and Genetic Engineering

Identifying transcript location in cells Identifying where specific RNAs occur within a cell or tissue has been limited by technology and imaging capabilities. Expansion microscopy has allowed for better visualization of small structures by expanding the tissues with a polymer- and hydrogel-based system. Alon et al. combined expansion microscopy with long-read in situ RNA sequencing, resulting in a more precise visualization of the location of specific transcripts. This method, termed “ExSeq” for expansion sequencing, was used to detect RNAs, both new transcripts and those previously demonstrated to localize to neuronal dendrites. Unlike other in situ sequencing methods, ExSeq does not target sets of genes. This technology thus unites spatial resolution, multiplexing, and an unbiased approach to reveal insights into RNA localization and its physiological roles in developing and active tissue. Science , this issue p. eaax2656