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Fusobacterium watanabei sp. nov. As additional species within the genus Fusobacerium, isolated from human clinical specimens

2021; Elsevier BV; Volume: 69; Linguagem: Inglês

10.1016/j.anaerobe.2021.102323

1095-8274

Junko Tomida, Toru Akiyama-Miyoshi, Kaori Tanaka, Masahiro Hayashi, Ryo Kutsuna, Nagatoshi Fujiwara, Yoshiaki Kawamura,

Bacterial Identification and Susceptibility Testing

Eight spindle-shaped bacteria were isolated from clinical samples in Japan and investigated for their taxonomic position. Phylogenetic trees (based on 16S rRNA, rpoB, zinc protease, and gyrB gene sequence comparisons) showed distinct clustering of eight strains with the type strain of Fusobacterium nucleatum and its closely related species. In silico whole genome comparison analysis based on average nucleotide index based on BLAST (ANIb) and digital DNA-DNA hybridization (dDDH) data between our clinical isolates (PAGU 1795, PAGU 1796T, and PAGU 1797) and the type strain of the closely related species showed values of less than 92.4% and 49.5%, respectively. On the basis of its phylogenetic and genomic distinctiveness together with differential phenotypic properties and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) characteristic signal patterns, we propose Fusobacterium watanabei sp. nov., with the type strain PAGU 1796T (= GTC 21791T = CCUG 74246T).