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Rhesus and cynomolgus macaque immunoglobulin heavy-chain genotyping yields comprehensive databases of germline VDJ alleles

2021; Cell Press; Volume: 54; Issue: 2 Linguagem: Inglês

10.1016/j.immuni.2020.12.018

1097-4180

Néstor Vázquez Bernat, Martin Corcoran, Izabela Nowak, Mateusz Kaduk, Xaquín Castro Dopico, Sanjana Narang, Pauline Maisonasse, Nathalie Dereuddre‐Bosquet, Ben Murrell, Gunilla B. Karlsson Hedestam,

Monoclonal and Polyclonal Antibodies Research

Definition of the specific germline immunoglobulin (Ig) alleles present in an individual is a critical first step to delineate the ontogeny and evolution of antigen-specific antibody responses. Rhesus and cynomolgus macaques are important animal models for pre-clinical studies, with four main sub-groups being used: Indian- and Chinese-origin rhesus macaques and Mauritian and Indonesian cynomolgus macaques. We applied the (Ig) gene inference tool IgDiscover and performed extensive Sanger sequencing-based genomic validation to define germline VDJ alleles in these 4 sub-groups, comprising 45 macaques in total. There was allelic overlap between Chinese- and Indian-origin rhesus macaques and also between the two macaque species, which is consistent with substantial admixture. The island-restricted Mauritian cynomolgus population displayed the lowest number of alleles of the sub-groups, yet maintained high individual allelic diversity. These comprehensive databases of germline IGH alleles for rhesus and cynomolgus macaques provide a resource toward the study of B cell responses in these important pre-clinical models.