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Nonenzymatic polymerase-like template-directed synthesis of acyclic l-threoninol nucleic acid

2021; Nature Portfolio; Volume: 12; Issue: 1 Linguagem: Inglês

10.1038/s41467-021-21128-0

2041-1723

Keiji Murayama, Hikari Okita, Takumi Kuriki, Hiroyuki Asanuma,

Bacterial Genetics and Biotechnology

Abstract Evolution of xeno nucleic acid (XNA) world essentially requires template-directed synthesis of XNA polymers. In this study, we demonstrate template-directed synthesis of an acyclic XNA, acyclic l -threoninol nucleic acid ( l - a TNA), via chemical ligation mediated by N -cyanoimidazole. The ligation of an l - a TNA fragment on an l - a TNA template is significantly faster and occurs in considerably higher yield than DNA ligation. Both l - a TNA ligation on a DNA template and DNA ligation on an l - a TNA template are also observed. High efficiency ligation of trimer l - a TNA fragments to a template-bound primer is achieved. Furthermore, a pseudo primer extension reaction is demonstrated using a pool of random l - a TNA trimers as substrates. To the best of our knowledge, this is the first example of polymerase-like primer extension of XNA with all four nucleobases, generating phosphodiester bonding without any special modification. This technique paves the way for a genetic system of the l - a TNA world.