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Saliva Pooling Strategy for the Large-Scale Detection of SARS-CoV-2, Through Working-Groups Testing of Asymptomatic Subjects for Potential Applications in Different Workplaces

2021; Lippincott Williams & Wilkins; Volume: 63; Issue: 7 Linguagem: Inglês

10.1097/jom.0000000000002176

1536-5948

Daniel Alberto Girón‐Pérez, Rocío Alejandra Ruiz‐Manzano, Alma Betsaida Benítez-Trinidad, Guadalupe Herminia Ventura‐Ramón, Carlos Eduardo Covantes‐Rosales, Ansonny Jhovanny Ojeda-Durán, Ulises Mercado‐Salgado, Gladys Alejandra Toledo‐Ibarra, Karina Janice Guadalupe Díaz‐Reséndiz, Manuel Iván Girón‐Pérez,

Dental Research and COVID-19

Objective: To perform an improved large-scale SARS-CoV-2 detection on pooled tests of asymptomatic workers. Methods: qRT-PCR validation of the SARS-CoV-2 detection in salivae samples and saliva pools and working-group saliva pooling and testing for SARS-CoV-2. Results: We found a high Cycle threshold correlation ( r = 0.9099) between swabs and saliva samples. Then, through the pooling strategy, we detected that 18/360 (5%) of individual saliva samples were SARS-CoV-2 positive. Saliva-pooling efficiency (360 of test sample/30 individual PCR) was higher (5.45) than the reported for swabbing group-testing and we spared 82% of the PCR reagents as well as sampling and personal protection equipment. Conclusion: Through this simplified and less expensive procedure, we detected in a short time asymptomatic-infected SARS-CoV-2-carriers that were isolated from their co-workers, thus, this methodology can be implemented in different workplaces to ensure consumers that employees are not infectious.