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Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis

2021; Springer Nature; Volume: 48; Issue: 5 Linguagem: Inglês

10.1007/s11033-021-06494-8

1573-4978

Zhangrui Cheng, Laura Buggiotti, Mazdak Salavati, Cinzia Marchitelli, Sergio Palma-Vera, A.R.G. Wylie, Haruko Takeda, Lijing Tang, M.A. Crowe, D.C. Wathes, M.A. Crowe, Niamh McLoughlin, Alan G. Fahey, Elizabeth Matthews, Andreia Santoro, C.J. Byrne, Pauline M. Rudd, Róisín O’Flaherty, Sinead Hallinan, Claire Wathes, Zhangrui Cheng, Ali Fouladi, Geoff Pollott, Dirk Werling, Beatriz Sanz‐Bernardo, Mazdak Salavati, Laura Buggiotti, A.R.G. Wylie, Matthew R. Bell, Mieke Vaneetvelde, Kristof Hermans, G. Opsomer, Sander Moerman, Jenne De Koster, Hannes Bogaert, Jan Vandepitte, Leila Vandevelde, Bonny Vanranst, Johanna K Höglund, Susanne Dahl, K.L. Ingvartsen, Martin Tang Sørensen, Leslie Foldager, Søren Østergaard, Janne Rothmann, Mogens Agerbo Krogh, Else Meyer, Charlotte Gaillard, Jehan Frans Ettema, Tine Rousing, Federica Signorelli, F. Napolitano, B. Moioli, Alessandra Crisà, L. Buttazzoni, Jennifer B. McClure, Daragh Matthews, F. Kearney, A.R. Cromie, Matt McClure, Shujun Zhang, Xing Chen, Huanchun Chen, Junlong Zhao, Liguo Yang, Guohua Hua, Tan Chen, Guiqiang Wang, M. Bonneau, Andrea Pompozzi, Armin Pearn, Arnold Evertson, Linda Kosten, Anders Fogh, Thomas Emil Andersen, M.C. Lucy, Christine G. Elsik, Gavin C. Conant, Jerry W. Taylor, Nicolas Gengler, Michel Georges, Frédéric Colinet, Marilou Ramos Pamplona, Hedi Hammami, Catherine Bastin, Haruko Takeda, Aurélie Laine, Anne-Sophie Van Laere, Martin Schulze, Cinzia Marchitelli, Sergio Palma-Vera,

Antimicrobial Peptides and Activities

Bovine mastitis, an inflammatory disease of the mammary gland, is classified as subclinical or clinical. Circulating neutrophils are recruited to the udder to combat infection. We compared the transcriptomic profiles in circulating leukocytes between healthy cows and those with naturally occurring subclinical or clinical mastitis. Holstein Friesian dairy cows from six farms in EU countries were recruited. Based on milk somatic cell count and clinical records, cows were classified as healthy (n = 147), subclinically (n = 45) or clinically mastitic (n = 22). Circulating leukocyte RNA was sequenced with Illumina NextSeq single end reads (30 M). Differentially expressed genes (DEGs) between the groups were identified using CLC Genomics Workbench V21, followed by GO enrichment analysis. Both subclinical and clinical mastitis caused significant changes in the leukocyte transcriptome, with more intensive changes attributed to clinical mastitis. We detected 769 DEGs between clinical and healthy groups, 258 DEGs between subclinical and healthy groups and 193 DEGs between clinical and subclinical groups. Most DEGs were associated with cell killing and immune processes. Many upregulated DEGs in clinical mastitis encoded antimicrobial peptides (AZU1, BCL3, CAMP, CATHL1, CATHL2, CATHL4,CATHL5, CATHL6, CCL1, CXCL2, CXCL13, DEFB1, DEFB10, DEFB4A, DEFB7, LCN2, PGLYRP1, PRTN3, PTX3, S100A8, S100A9, S100A12, SLC11A1, TF and LTF) which were not upregulated in subclinical mastitis. The use of transcriptomic profiles has identified a much greater up-regulation of genes encoding antimicrobial peptides in circulating leukocytes of cows with naturally occurring clinical compared with subclinical mastitis. These could play a key role in combatting disease organisms.