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Comparison between cultured and wild Pacific white shrimp ( Penaeus vannamei ) vitellogenesis: next-generation sequencing and relative expression of genes directly and indirectly related to reproduction

2021; PeerJ, Inc.; Volume: 9; Linguagem: Inglês

10.7717/peerj.10694

2167-8359

Araceli Lorena Montes-Dominguez, Jesus Arian Avena-Soto, Jorge Luis Lizarraga-Rodriguez, Rodrigo de Jesus Perez-Gala, Stephanie Jimenez-Gutierrez, Jesus Alberto Sotelo-Falomir, Fernando Marino Pinzon-Miranda, Francisco Martínez‐Pérez, Horacio A. Muñoz-Rubí, Darío Chávez-Herrera, Laura Rebeca Jiménez-Gutiérrez,

Marine Bivalve and Aquaculture Studies

Shrimp fisheries are among the most important fisheries worldwide, and shrimp culture has increased considerably in recent years. Most current studies on reproduction-related genes have been conducted on cultured shrimp. However, gene expression is intimately linked to physiological and environmental conditions, and therefore an organism’s growth environment has a great influence on reproduction. Thus, gene expression profiling, should be applied in fisheries studies. Here, we identified the expression patterns of 76 reproduction-related genes in P. vannamei via the analysis of pooled transcriptomes from a time-series experiment encompassing a full circadian cycle. The expression patterns of genes associated both directly ( Vtg , ODP , and ProR ) and indirectly ( FAMet , CruA1 , and CruC1 ) with reproduction were evaluated, as these genes could be used as molecular markers of previtellogenic and vitellogenic maturation stages. The evaluated genes were prominently upregulated during vitellogenic stages, with specific expression patterns depending on the organism’s environment, diet, and season. Vtg , ProR , ODP , and FaMet could serve as molecular markers for both wild and cultured organisms.