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Induction of Immunogenic Cell Death of Esophageal Squamous Cell Carcinoma by 5-Fluorouracil and Cisplatin

2021; Stanford University Highwire Press; Volume: 35; Issue: 2 Linguagem: Inglês

10.21873/invivo.12315

1791-7549

Junya Nishimura, Sota Deguchi, Hiroaki Tanaka, Yoshihito Yamakoshi, Mami Yoshii, Tatsuro Tamura, Takahiro Toyokawa, Shigeru Lee, Kazuya Muguruma, Masaichi Ohira,

Adenosine and Purinergic Signaling

Background/Aim: Neoadjuvant chemotherapy (NAC) using 5-FU (5-fluorouracil)/CDDP (cisplatin) is a standard therapy for stage II/III thoracic esophageal squamous cell carcinoma (ESCC) in Japan. The aim of this study was to investigate whether 5-FU/CDDP could induce immunogenic cell death in ESCC cell lines. Materials and Methods: Tumor samples for immunohistochemistry were obtained from 50 patients (mean age=63.1 years) with pathological stage 0-IVa ESCC who underwent NAC followed by surgery. Cell lines T.T and KYSE30 were used for the in vitro experiments. Results: The concentrations of HMGB1 were elevated in the cell line supernatants treated with 5-FU/CDDP. 5-FU/CDDP treated dendritic cells (DCs) showed a mature phenotype, and enhanced T cell proliferation capacity. In addition, mature DCs were observed in surgical specimens with a histological response after treatment with 5-FU/CDDP chemotherapy. Conclusion: 5-FU/CDDP could induce immunogenic cell death in the tumor microenvironment of ESCC.