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A sugarcane cystatin (CaneCPI-5) alters microcosm biofilm formation and reduces dental caries

2021; Taylor & Francis; Volume: 37; Issue: 1 Linguagem: Inglês

10.1080/08927014.2021.1881065

1029-2454

Tamara Teodoro Araújo, Gabriel Domingues Camiloti, Aline Dionízio, Natara Dias Gomes da Silva, Beatriz Martines de Souza, Thamyris de Souza Carvalho, João Víctor Frazão Câmara, Priscila Yumi Tanaka Shibao, Flávio Henrique‐Silva, Thiago Cruvinel, Ana Carolina Magalhães, Marília Afonso Rabelo Buzalaf,

Dental Erosion and Treatment

The antimicrobial and anticaries effects of CaneCPI-5 were evaluated. Ninety bovine enamel samples were treated for 60 s with either phosphate-buffered-saline (PBS), 0.12% chlorhexidine (CHX), 0.05 mg ml−1 CaneCPI-5, 0.1 mg ml−1 CaneCPI-5 or 0.5 mg ml−1 CaneCPI-5. They were incubated with inoculum (human saliva + McBain's saliva) for the first 8 h. From then until the end of the experiment, the enamel was exposed to McBain saliva with sucrose and, once a day, for 5 days, they were treated with the solutions. At the end of the experimental period, resazurin and viable plate count assays were performed. Enamel demineralization was also measured. All concentrations of CaneCPI-5 and CHX significantly reduced the activity of biofilms compared with PBS. For viable plate counts, all treatments similarly reduced the lactobacilli and total streptococci; for the mutans streptococci, 0.05 mg ml−1 CaneCPI-5 performed better than CHX. All CaneCPI-5 concentrations significantly reduced the integrated mineral loss. This study represents the first step regarding the use of CaneCPI-5 within the concept of acquired enamel pellicle and biofilm engineering to prevent dental caries.