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STUDY OF THE EFFECT OF PARACETAMOL BINDED IN POLYMERic NANOPARTICLES ON DAFNIA MAGNA

2020; Volume: 2020; Linguagem: Inglês

10.37904/nanocon.2020.3747

2694-930X

Barbora Havelkova, Karel Sehnal, Dominik Banas, Marta Kepinská, Branislav Ruttkay-Nedecký, M. Staňková, Carlos Fernández, Miroslava Beklová, René Kizek,

Medicinal Plants and Neuroprotection

Drugs are important xenobiotics in the environment.Their use increases with the growth of the human population, but also in agricultural primary production.Paracetamol (PAR) is a widely used analgesic and antipyretic and its production is still growing.Commonly available drug production technologies are being developed very intensively with nanotechnological modifications for their gradual and targeted release.Nanoparticles (ST/PAR) from starch were prepared: PAR (0, 1, 2, 3, 5 and 10 mg/L) was mixed with citric acid ester in a 1:8 v/v ratio for 30 min at 25 ⁰C.By the centrifugation (16.000 g, 30 min) ST/PAR were obtained in the pellet.The effect of PAR was studied on Daphnia magna Straus (Cladocera, Crustacea).Adult females (70-400 mg) were used for self-evaluation.The EC50 was 3.749 mg/L after 48 h of PAR treatment.Total protein values determined by Lowry method were between 0.5-2.2mg/mL and by Bradford method between 190-676 mg/L.Antioxidant activity values determined by CUPRAC method were between 4-15 µg/mL GAE and by ABTS method ranged between 40-103 µg/mL GAE.PAR values were between 9-40 µM.Subsequently, the biological activity of the prepared nanoparticles was tested.