A dansyl-derivatized phytic acid analogue as a fluorescent substrate for phytases: experimental and computational approach
2021; Elsevier BV; Volume: 110; Linguagem: Inglês
10.1016/j.bioorg.2021.104810
ISSN1090-2120
AutoresChristophe Dussouy, Éric Dubreucq, Patrick Chemardin, Véronique Perrier, Josiane Abadie, Hervé Quiquampoix, Claude Plassard, Jean‐Bernard Behr,
Tópico(s)Oral and gingival health research
ResumoA new myo-inositol pentakisphosphate was synthesized, which featured a dansyl group at position C-5.The fluorescent tag was removed from the inositol by a 6-atom spacer to prevent detrimental steric interactions in the catalytic site of phytases.The PEG linker was used in order to enhance hydrophilicity and biocompatibility of the new artificial substrate.Computational studies showed a favorable positioning in the catalytic site of phytases.Enzymatic assays demonstrated that the tethered myo-inositol was processed by two recombinant phytases Phy-A and Phy-C, classified
Referência(s)