Discovery of a SARS-CoV-2 variant from the P.1 lineage harboring K417T/E484K/N501Y mutations in Kofu, Japan
2021; Elsevier BV; Volume: 82; Issue: 6 Linguagem: Inglês
10.1016/j.jinf.2021.03.013
ISSN1532-2742
Autores Tópico(s)Viral gastroenteritis research and epidemiology
Resumo•SARS-CoV-2 genomic surveillance reveals the prevailing lineage in Japan.•Several strains were identified, from the original one to the P.1 lineage in Brazil.•This is the first report identifying P.1 lineage (20 J/501Y.V3) in the city, Japan. In this Journal, Tang and colleagues recently commented on the introduction of the South African SARS-CoV-2 variant 501Y.V2 into the United Kingdom1Tang J.W., Toovey O.T.R., Harvey K.N., Hui D.D.S.: Introduction of the South African SARS-CoV-2 variant 501Y.V2 into the UK. J. Infect.Google Scholar. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are not always related to increased threat to human health because the virus acquires genomic diversity during the course of its life cycle2van Dorp L. Richard D. Tan C.C.S. Shaw L.P. Acman M. Balloux F. No evidence for increased transmissibility from recurrent mutations in SARS-CoV-2.Nat. Commun. 2020; 11: 5986Crossref PubMed Scopus (174) Google Scholar. However, some of these mutations have been shown to be associated with attenuation of the neutralizing activity of antibodies3Wang Z. Schmidt F. Weisblum Y. Muecksch F. Barnes C.O. Finkin S. Schaefer-Babajew D. Cipolla M. Gaebler C. Lieberman J.A. et al.mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.Nature. 2021; Crossref Scopus (886) Google Scholar. During the ongoing evolution of SARS-CoV-2, newly emerging lineages are likely to be circulating in the human population and genomic surveillance will be important for evaluating the emergence, spread, vaccine efficacy, and transmissibility of these lineages. Currently, an emergent D614G mutation in the spike glycoprotein of SARS-CoV-2 is prevalent globally4Korber B. Fischer W.M. Gnanakaran S. Yoon H. Theiler J. Abfalterer W. Hengartner N. Giorgi E.E. Bhattacharya T. Foley B. et al.Tracking Changes in SARS-CoV-2 Spike: evidence that D614G Increases Infectivity of the COVID-19 Virus.Cell. 2020; Abstract Full Text Full Text PDF PubMed Scopus (2639) Google Scholar. More recently, new emerging lineages with spike protein mutations have been discovered in the United Kingdom (B.1.1.7 lineage, 20I/501Y.V1, also named VOC 202,012/01)5European Centre for Disease Prevention and Control: Rapid increase of a SARS-CoV-2 variant with multiple spike protein mutations observed in the United Kingdom. 2020:1–13.Google Scholar, South Africa (B.1.351 lineage, 20H/501Y.V2)6Tegally H. Wilkinson E. Giovanetti M. Iranzadeh A. Fonseca V. Giandhari J. Doolabh D. Pillay S. San E.J. Msomi N. et al.Emergence and rapid spread of a new severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) lineage with multiple spike mutations in South Africa.medRxiv. 2020; 2020 (2012.2021.20248640)Google Scholar, and Brazil (P.1 lineage, 20 J/501Y.V3)7Faria NR. Claro I.M. Candido D. Moyses Franco LA. Andrade PS. Coletti TM. Silva CA.M. Sales FC. Manuli ER. Aguiar RS. et al.Genomic characterisation of an emergent SARS-CoV-2 lineage in Manaus: preliminary findings.Virological. 2020; https://virological.org/t/genomic-characterisation-of-an-emergent-sars-cov-2-lineage-in-manaus-preliminary-findings/586Google Scholar,8Naveca F, Nascimento V, Souza V, Corado A, Nascimento F, Silva G, Costa Á, Duarte D, Pessoa K, Gonçalves L, et al. Phylogenetic relationship of SARS-CoV-2 sequences from Amazonas with emerging Brazilian variants harboring mutations E484K and N501Y in the Spike protein. https://virologicalorg/t/phylogenetic-relationship-of-sars-cov-2-sequences-from-amazonas-with-emerging-brazilian-variants-harboring-mutations-e484k-and-n501y-in-the-spike-protein/585 2021.Google Scholar. All of these lineages have a N501Y mutation in the receptor binding domain (RBD), which directly binds to the angiotensin converting enzyme 2 (ACE2) receptor of the host cell, contributing to increased transmissibility. Both B.1.351 and P.1 lineages also have additional K417N/T and E484K mutations. K417N/T, E484K, and N501Y confer reduced neutralizing activity of convalescent and mRNA vaccine-elicited serum3Wang Z. Schmidt F. Weisblum Y. Muecksch F. Barnes C.O. Finkin S. Schaefer-Babajew D. Cipolla M. Gaebler C. Lieberman J.A. et al.mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.Nature. 2021; Crossref Scopus (886) Google Scholar. To determine the genomic characteristics of the SARS-CoV-2 variant identified in the Kofu, Japan, we started whole genome sequencing analysis using the Ion Torrent Genexus System (Thermo Fisher Scientific) on January 8, 2021. By February 15, 2021, a total of 136 samples from COVID-19-positive patients were obtained in our hospital from which 70 were subjected to analysis9Hirotsu Y. Maejima M. Shibusawa M. Nagakubo Y. Hosaka K. Amemiya K. Sueki H. Hayakawa M. Mochizuki H. Tsutsui T. et al.Pooling RT-qPCR testing for SARS-CoV-2 in 1000 individuals of healthy and infection-suspected patients.Sci. Rep. 2020; 10: 18899Crossref PubMed Scopus (27) Google Scholar,10Hirotsu Y. Maejima M. Shibusawa M. Amemiya K. Nagakubo Y. Hosaka K. Sueki H. Hayakawa M. Mochizuki H. Tsutsui T. et al.Prospective study of 1,308 nasopharyngeal swabs from 1,033 patients using the LUMIPULSE SARS-CoV-2 antigen test: comparison with RT-qPCR.Int. J. Infect. Dis. 2021; Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar. The sequence data were subjected to phylogenetic analysis using Nextclade, identifying five types of clades: 19A (n = 1), 20A (n = 3), 20B (n = 59), 20C (n = 6), and 20 J/501Y.V3 (n = 1) (Fig. 1). The SARS-CoV-2 strain from the Diamond Princess cruise ship patient was classified into clade 19A (Fig. 1), and four patients admitted at the end of March 2020 were classified into 20A (n = 3) and 20B (n = 1). For the patients admitted from September 2020–January 2021, 64 patients were classified into 20B clade (n = 58) and 20C (n = 6) (Fig. 1). The newly confirmed patient was classified as 20 J/501Y.V3 (P.1 lineage) on February 10, 2021 (Accession No. EPI_ISL_978917). This patient was a 46-year-old man who entered our hospital in early February 2021 with a fever at 38.9 °C and with a history of staying in Brazil. RT-qPCR indicated a high viral load (7.1 log10/µL) and low cycle threshold (Ct) value of 15. The patient had displayed mild symptoms upon returning to Japan 4 days earlier and was admitted to another hospital. However, he was declared negative for SARS-CoV-2 during quarantine. Sequencing analysis revealed that the SARS-CoV-2 variant 20 J/501Y.V3 (P.1 lineage) had 37 mutations, including 22 missense, 10 synonymous, three intergenic, one frameshift, and one in-frameshift mutation. In the spike protein, we observed 12 missense mutations (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I and V1176F). These mutations matched perfectly with the mutations in the P.1 variant previously discovered in Brazil7Faria NR. Claro I.M. Candido D. Moyses Franco LA. Andrade PS. Coletti TM. Silva CA.M. Sales FC. Manuli ER. Aguiar RS. et al.Genomic characterisation of an emergent SARS-CoV-2 lineage in Manaus: preliminary findings.Virological. 2020; https://virological.org/t/genomic-characterisation-of-an-emergent-sars-cov-2-lineage-in-manaus-preliminary-findings/586Google Scholar (Fig. 2A). In the RBD of the spike protein, three mutations (K417T, E484K and N501Y) were identified. These results indicated that we had identified a variant related to 20 J/501Y.V3 (P.1 lineage) in Japan. To examine the global prevalence of 20 J/501Y.V3 (P.1 lineage), we referred to sequence data deposited in GISAID (https://www.gisaid.org/). By February 14, 2021, 121 sequence data were available, 119 of which were derived from patients. Variant 20 J/501Y.V3 (P.1 lineage) was first discovered in a sample collected from Manaus, Amazonas, Brazil on December 4, 2020, and has subsequently been identified from numerous other samples (Fig 2B). Almost all 20 J/501Y.V3 (P.1 lineage) samples sequenced have 33–40 mutations compared with original strain reported from Wuhan, China; our identified strain has 37 mutations (Fig. 2C). Of the 119 patients infected with this variant, 82 (68.9%) were identified in Brazil, five (4.2%) in Japan, 20 (16.8%) in Europe, and three (2.5%) in the USA (Supplemental Table 1), suggesting global prevalence. In summary, we have confirmed the emergence of five clades over time. Consecutive analysis identified SARS-CoV-2 variant 20 J/501Y.V3 (P.1 lineage) in a patient and detected mutations that are identical to those of the original P.1 variant discovered in Brazil7Faria NR. Claro I.M. Candido D. Moyses Franco LA. Andrade PS. Coletti TM. Silva CA.M. Sales FC. Manuli ER. Aguiar RS. et al.Genomic characterisation of an emergent SARS-CoV-2 lineage in Manaus: preliminary findings.Virological. 2020; https://virological.org/t/genomic-characterisation-of-an-emergent-sars-cov-2-lineage-in-manaus-preliminary-findings/586Google Scholar. This is the first report of 20 J/501Y.V3 (P.1 lineage) in Kofu, Japan. None. This study was supported by a Grant-in-Aid for the Genome Research Project from Yamanashi Prefecture (to M.O. and Y.H.), the Japan Society for the Promotion of Science (JSPS) KAKENHI Early-Career Scientists JP18K16292 (to Y.H.), a Grant-in-Aid for Scientific Research (B) 20H03668 (to Y.H.), a Research Grant for Young Scholars (to Y.H.), the YASUDA Medical Foundation (to Y.H.), the Uehara Memorial Foundation (to Y.H.), and Medical Research Grants from the Takeda Science Foundation (to Y.H.).
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