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Injectable in situ forming hydrogels incorporating dual-nanoparticles for chemo-photothermal therapy of breast cancer cells

2021; Elsevier BV; Volume: 600; Linguagem: Inglês

10.1016/j.ijpharm.2021.120510

1873-3476

Ivo J. Sabino, Rita Lima‐Sousa, Cátia G. Alves, Bruna L. Melo, André F. Moreira, Ilídio J. Correia, Duarte de Melo‐Diogo,

Nanoparticle-Based Drug Delivery

Chemo-photothermal therapy (chemo-PTT) mediated by nanomaterials holds a great potential for cancer treatment. However, the tumor uptake of the systemically administered nanomaterials was recently found to be below 1%. To address this limitation, the development of injectable tridimensional polymeric matrices capable of delivering nanomaterials directly into the tumor site appears to be a promising approach. In this work, an injectable in situ forming ionotropically crosslinked chitosan-based hydrogel co-incorporating IR780 loaded nanoparticles (IR/BPN) and Doxorubicin (DOX) loaded nanoparticles (DOX/TPN) was developed for application in breast cancer chemo-PTT. The produced hydrogels (IR/[email protected] and IR/BPN+DOX/[email protected]) displayed suitable physicochemical properties and produced a temperature increase of about 9.1 °C upon exposure to Near Infrared (NIR) light. As importantly, the NIR-light exposure also increased the release of DOX from the hydrogel by 1.7-times. In the in vitro studies, the combination of IR/[email protected] with NIR light (photothermal therapy) led to a reduction in the viability of breast cancer cells to 35%. On the other hand, the non-irradiated IR/BPN+DOX/[email protected] (chemotherapy) only diminished cancer cells' viability to 85%. In contrast, the combined action of IR/BPN+DOX/[email protected] and NIR light reduced cancer cells' viability to about 9%, demonstrating its potential for breast cancer chemo-PTT.