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Knockdown of circ_NEK6 Decreased 131 I Resistance of Differentiated Thyroid Carcinoma via Regulating miR-370-3p/MYH9 Axis

2021; SAGE Publishing; Volume: 20; Linguagem: Inglês

10.1177/15330338211004950

1533-0346

Fukun Chen, Shuting Yin, Zhiping Feng, Chao Liu, Juan Lv, Yuanjiao Chen, Ruoxia Shen, Jiaping Wang, Zhiyong Deng,

Fatigue and fracture mechanics

Radioresistance is a crucial factor for the failure of iodine 131 ( 131 I)-based radiotherapy for differentiated thyroid carcinoma (DTC). This study aimed to explore the effect of circ_NEK6 on the development of 131 I resistance in DTC and its potential mechanism. In this study, we demonstrated that circ_NEK6 expression was significantly elevated in 131 I-resistant DTC tissues and cell lines. Knockdown of circ_NEK6 significantly repressed 131 I resistance via inhibiting cell proliferation, migration, invasion abilities, and inducing cell apoptosis and DNA damage in 131 I-resistant DTC cells. Mechanistically, knockdown of circ_NEK6 suppressed 131 I resistance in DTC by upregulating the inhibitory effect of miR-370-3p on the expression of myosin heavy chain 9 (MYH9). In vivo experiments showed that circ_NEK6 inhibition aggravated 131 I radiation-induced inhibition of xenograft tumor growth. Taken together, knockdown of circ_NEK6 repressed 131 I resistance in DTC cells by regulating the miR-370-3p/MYH9 axis, indicating that circ_NEK6 may act as a potential biomarker and therapeutic target for DTC patients with 131 I resistance.