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Erratum to: A novel deconvolution method for modeling UDP-N-acetyl-D-glucosaminebiosynthetic pathways based on 13C mass isotopologue profiles undernon-steady-state conditions

2012; BioMed Central; Volume: 10; Issue: 1 Linguagem: Inglês

10.1186/1741-7007-10-74

ISSN

1741-7007

Autores

Hunter Moseley, Andrew N. Lane, Alex Belshoff, Richard M. Higashi, Teresa W.‐M. Fan,

Tópico(s)

Mitochondrial Function and Pathology

Resumo

Figure 1 Species assignments of UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) isotopologues in Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS).The same crude extracts used for NMR were analyzed following re-exchange of 2 H back to 1 H. Analysis conditions are stated in the text.With correction to an internal reference, all of the isotopologues were assignable at better than 1 ppm mass accuracy, with most better than 10 ppb mass accuracy.The molecular formulae were assigned using Xcalibur software with elemental limits set to CHONP and allowing up to 17 occurrences of 13 C.The combination of the ultra-high resolution with extreme mass accuracy resulted in high confidence that only 'pure' 13 C isotopologues were quantified for the moiety modeling.

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