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Effects of cytokine gene therapy on prolonging survival the of allografted skin after scalding in a murine model

2000; Chinese Medical Association; Linguagem: Inglês

1009-2587

Gu Caizhi,

Wound Healing and Treatments

Objective To explore the role of cytokine gene therapy on prolonging survival time of allografted skin after scalding in a muine model. Methods Interleukin 10 (IL-10) gene was employed as therapeutic objective gene and fibroblast was as a carrier cell. Gene transcription technique was adopted to establish an experimental murine model in which fibroblast-mediated gene therapy was used to prolong allografted skin survival time after scalding. IL-10 was transferered into fibroblastocyte (NIH3T3) by reverse transcriptive virus vector. The mice were grafted with allosikin after scalding. In addition, collagen capsulized NIH3T-3-IL-10 cells were implanted intraperitoneally in the mice so as to observe its influence on allografted skin survival time and on the changes of their main internal organs. Results Cytokine gene therapy could obviously prolong the survival time of allografted skin (P 0.01) without any evident detrimental effect on the internal organs. Conclusion These results demonstrated that skin allograft rejection could be inhibited and the survival time be prolonged with the implantation of the fibroblastocyte transferred IL-10 gene.