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Green Fabrication of (6,5)Carbon Nanotube/Protein Transistor Endowed with Specific Recognition

2021; Wiley; Volume: 7; Issue: 5 Linguagem: Inglês

10.1002/aelm.202001114

2199-160X

Marcello Berto, Matteo Di Giosia, Martina Giordani, Matteo Sensi, Francesco Valle, Andrea Alessandrini, Claudia Menozzi, Andrea Cantelli, Gian Carlo Gazzadi, Francesco Zerbetto, Matteo Calvaresi, Fabio Biscarini, Carlo Augusto Bortolotti,

Neuroscience and Neural Engineering

Abstract A general single‐step approach is introduced for the green fabrication of hybrid biosensors from water dispersion. The resulting device integrates the semiconducting properties of a carbon nanotube (CNT) and the functionality of a protein. In the initial aqueous phase, the protein (viz., lysozyme [LZ]) disperses the (6,5)CNT. Drop‐casting of the dispersion on a test pattern (a silicon wafer with interdigitated Au source and drain electrodes) yields a fully operating, robust, electrolyte‐gated transistor (EGT) in one step. The EGT response to biorecognition is then assessed using the LZ inhibitor N ‐acetyl glucosamine trisaccharide. Analysis of the output signal allows one to extract a protein‐substrate binding constant in line with values reported for the free (without CNT) system. The methodology is robust, easy to optimize, redirectable toward different targets and sets the grounds for a new class of CNT‐protein biosensors that overcome many limitations of the technology of fabrication of CNT biosensors.