Analysis of Differentially Expressed Proteome in Urinary Exosome from Non-small Cell lung Cancer Patients
2010; Elsevier BV; Linguagem: Inglês
ISSN
0253-3820
Autores Tópico(s)Hepatitis C virus research
ResumoUrine provides an alternative to blood plasma as a potential source of disease biomarkers. Exosomes was separated by ultracentrifuge at 200000 g in normal persons and non-small cell lung cancer ( NSCLC) patients′ urine. For proteomic analysis of urinary exosome,1D sodium dodecylsulfonate-polyacrylate gel electrophoresis( SDS-PAGE) was carries out and cut the gel 31 kDa-20 kDa bands in normal group and disease group′s. These gel blocks were subjected to in-gel trypsinization,and the extracted peptides were analyzed HPLC-CHIP-MS/MS. Approximately 24 unique proteins were identified in the UniProtKB/SWISSPORT. The difference expression proteins were found in urinary exosome from NSCLC patients,including three fragment of the immunoglobulin kappa,two kinds of Ras related proteins,glutathione S-transferase A2, serum amyloid P-component precursor and phosphatidylethanolamine-binding protein 1.
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