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First Report of Leaf Spot of Lily Caused by Epicoccum sorghinum in China

2018; American Phytopathological Society; Volume: 102; Issue: 12 Linguagem: Inglês

10.1094/pdis-05-18-0787-pdn

1943-7692

Huilan Zeng, Qianyi Lu, R. Li,

Plant Pathogens and Resistance

HomePlant DiseaseVol. 102, No. 12First Report of Leaf Spot of Lily Caused by Epicoccum sorghinum in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Leaf Spot of Lily Caused by Epicoccum sorghinum in ChinaH. Zeng, Q. Lu, and R. LiH. Zenghttp://orcid.org/0000-0001-7723-0486Search for more papers by this author, Q. LuSearch for more papers by this author, and R. Li†Corresponding author: R. Li; E-mail: E-mail Address: 13507058200@163.comSearch for more papers by this authorAffiliationsAuthors and Affiliations H. Zeng Q. Lu R. Li † , Department of Horticulture, College of Life Science and Environmental Resource, Yichun University, Yichun 336000, Jiangxi Province, China. Published Online:27 Sep 2018https://doi.org/10.1094/PDIS-05-18-0787-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Lilium brownii var. viridulum (Liliaceae) is grown as an edible bulbiferous plant, and it has been cultivated extensively in central China. In 2016, drying of lily leaf tip was observed in 30 to 50% of 15- to 20-day-old plants with a surveyed area of 1.5 ha in commercial fields located in Wanzai County, Yichun, Jiangxi Province, China. Affected leaves showed water-soaked, chlorotic spots that coalesced to form irregular light-brown necrotic spots with many black pycnidia in the mesophyll tissues as the disease progressed. Five symptomatic leaf samples were surface sterilized with 70% ethanol for 1 min and 0.1% mercuric chloride (HgCl2) for 30 s and then washed thrice with sterilized distilled water. Samples (1 × 1 cm) were plated on potato dextrose agar (PDA) medium and incubated at 28°C in darkness (Liu et al. 2018). After 5 days, fungal colonies expressed on PDA were subcultured (Dhingra and Sinclair 1995). After 7 days of incubation, fungal colonies were observed for micromorphological characteristics. The fungal colonies were villose and regular and showed 42.95 cm after 5 days of inoculation. The pycnidia were produced on PDA medium and varied from 140.16 to 231.44 × 153.12 to 290.27 μm in diameter. Conidia were hyaline, unicellular, and elliptic to oval (0.9 to 3.0 × 1.0 μm). After 30 days of incubation on PDA, brown chlamydospores (5.98 to 13.33 × 4.19 to 7.48 μm) were seen and were spherical, having verrucose surface. Micromorphological characteristics of the fungus were identical to those of Epicoccum sorghinum (Liu et al. 2018). Further, the genomic DNA was extracted using the cetyl trimethyl ammonium bromide method, and the sequences of internal transcribed spacer (ITS), large subunit ribosomal rRNA (LSU), tubulin (TUB), and actin (ACT) were amplified and sequenced (Aveskamp et al. 2009; Lumbsch et al. 2000). The BLAST results showed that the LSU gene sequence (MH071388) was 99.93% homologous to sequences of E. sorghinum (GU237979), the ITS sequence (MH071389) was 99.81% similar to E. sorghinum (KJ767080), the ACT gene sequence (MH141601) was 100.00% homologous to E. sorghinum (KU728639), and the TUB gene sequence (MH141600) was 100.00% similar to E. sorghinum (KU728638). All the sequences for analysis were retrieved from GenBank of NCBI. Pathogenicity tests were performed on healthy leaves by inoculation with 5-mm mycelia discs of 7-day-old H10 fungal isolate and spore suspension. Three healthy leaves inoculated with sterile PDA discs immersed in distilled water served as controls and were incubated at 25°C with 90% relative humidity conditions, and the experiments were repeated four times (Liu et al. 2018). The first lesions were observed 2 days after inoculation, and significantly water-soaked necrotic lesions were found 5 days after inoculation. No disease symptoms were seen on control plants. The pathogen was reisolated from all inoculated plants, and the pathogen’s identity was confirmed. To our knowledge, this is the first report of E. sorghinum on L. brownii var. viridulum in China. Infection of lily leaf by E. sorghinum may cause losses in production fields in China. The potential impact of the pathogen on production must be considered in the implementation of disease control measures.References:Aveskamp, M., et al. 2009. Mycologia 101:363. https://doi.org/10.3852/08-199 Crossref, ISI, Google ScholarDhingra, D., and Sinclair, J. B. 1995. Basic Plant Pathology Methods. CRC, Boca Raton, FL. Google ScholarLiu, P., et al. 2018. Plant Dis. 102:682. https://doi.org/10.1094/PDIS-11-16-1621-PDN Link, ISI, Google ScholarLumbsch, H., et al. 2000. Plant Biol. 2:525. https://doi.org/10.1055/s-2000-7472 Crossref, ISI, Google ScholarFunding: This work is supported by the Key Research and Development Program of Science and Technology Agency of Jiangxi Province (grant no. 20161BBF60040).DetailsFiguresLiterature CitedRelated Vol. 102, No. 12 December 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Nov 2018Published: 27 Sep 2018First Look: 12 Jun 2018Accepted: 10 Jun 2018 Pages: 2648-2648 Information© 2018 The American Phytopathological SocietyFundingKey Research and Development Program of Science and Technology Agency of Jiangxi ProvinceGrant/Award Number: 20161BBF60040Cited byEpicoccum spp. Causing Maize Leaf Spot in Heilongjiang Province, ChinaXi Xu, Jingjing Li, Xilang Yang, Li Zhang, Shuo Wang, Guijin Shen, Bing Hui, Jialei Xiao, Changjian Zhou, Xiangjing Wang, Junwei Zhao, and Wensheng Xiang21 November 2022 | Plant Disease, Vol. 106, No. 12Sequence Data, Functional Annotation, and Relationship Analysis Between mRNAs and Long Noncoding RNAs from Tea Leaves During Infection by the Fungal Pathogen Epicoccum sorghinumChen Huang, Hongke Huang, Zhongqiu Xia, Yuqin Yang, Xinyue Jiang, Yuanyou Yang, Delu Wang, Xiangyang Li, and Zhuo Chen15 September 2022 | Molecular Plant-Microbe Interactions, Vol. 35, No. 9Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna1 January 2022 | Acta Botanica Brasilica, Vol. 36Epicoccum species: ubiquitous plant pathogens and effective biological control agents22 January 2021 | European Journal of Plant Pathology, Vol. 159, No. 4First Report of Leaf Spot Disease on Rice Caused by Epicoccum sorghinum in ChinaL. 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