Produção Nacional
Revisado por pares
Antimicrobial effects of photodynamic therapy with Fotoenticine on Streptococcus mutans isolated from dental caries
2021; Elsevier BV; Volume: 34; Linguagem: Inglês
10.1016/j.pdpdt.2021.102303
ISSN1873-1597
AutoresMaíra Terra Garcia, Cheyenne Marçal de Souza, Nathália Maria Ferreira Gonçalves, André Henrique Correia Pereira, Patrícia Pimentel de Barros, Alessandra Bühler Borges, Walter Miyakawa, Juliana Ferreira‐Strixino, Juliana Campos Junqueira,
Tópico(s)Nanoplatforms for cancer theranostics
ResumoPhotodynamic therapy (PDT) is a promising strategy to control cariogenic pathogens, such as Streptococcus mutans. Seeking to reach the total bacterial elimination from dental surfaces, novel photosensitizers have been investigated, such as Fotoenticine (FTC) derived from chlorin e6. The objective of this study was to investigate the photodynamic effects of FTC against several clinical strains of S. mutans. Clinical isolates were obtained from patients with active carious lesions, identified by molecular analysis and subjected to PDT using laser irradiation (660 nm and 39.5 J/cm2) in planktonic and biofilm stages. We identified 11 S. mutans strains from cervical, occlusal and proximal caries. PDT mediated by FTC has totally eliminated the S. mutans cells in planktonic growth for all analyzed strains. In biofilms, PDT with FTC reached statistically significant reductions compared with the non-treated control group, at 5.4, 5.5 and 6.5 Log10 (CFU/mL), respectively, for the strains from proximal, occlusal and cervical caries. The scanning electron microscopy evaluations confirmed that PDT mediated by FTC was able to disaggregate and kill the S. mutans cells adhered to enamel surface, suggesting its potential to disinfect the dental tissues.
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