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Molecular Detection of Drug-Resistance Genes of blaOXA-23-blaOXA-51 and mcr-1 in Clinical Isolates of Pseudomonas aeruginosa

2021; Multidisciplinary Digital Publishing Institute; Volume: 9; Issue: 4 Linguagem: Inglês

10.3390/microorganisms9040786

ISSN

2076-2607

Autores

Fabiana Nitz, Bruna Oliveira de Melo, Luís Cláudio Nascimento da Silva, Andrea de Souza Monteiro, Sirlei Garcia Marques, Valério Monteiro‐Neto, Rosimary de Jesus Gomes Turri, Antônio Dantas Silva, Patrícia Cristina Ribeiro Conceição, Hilário José Cardoso Magalhães, Adrielle Zagmignan, Thiago Azevedo Feitosa Ferro, Maria Rosa Quaresma Bomfim,

Tópico(s)

Pharmaceutical and Antibiotic Environmental Impacts

Resumo

Pseudomonas aeruginosa has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of P. aeruginosa recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among P. aeruginosa isolates. The drug susceptibility profiles of P. aeruginosa for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (mcr-1) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (blaOXA-23 and blaOXA-51) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of blaOXA-23 and blaOXA-51 genes in P. aeruginosa.

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