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Biohydrogen production from thermochemically pretreated corncob using a mixed culture bioaugmented with Clostridium acetobutylicum

2021; Elsevier BV; Volume: 46; Issue: 51 Linguagem: Inglês

10.1016/j.ijhydene.2021.04.046

1879-3487

Miguel A. Medina-Morales, Luis E. De la Cruz-Andrade, Lizeth A. Paredes-Peña, Thelma K. Morales-Martínez, José A. Rodríguez-De la Garza, I.M.M. Moreno-Dávila, María Concepción Tamayo‐Ordoñez, Leopoldo J. Ríos-González,

Biodiesel Production and Applications

Bovine ruminal fluid (BRF) bioaugmented with Clostridium acetobutylicum (Clac) was assessed for hydrolyzing cellulose and produce biohydrogen (BioH2) simultaneously from pretreated corncob in a single step, without the use of external hydrolytic biocatalysts. The corncob was pretreated using three thermochemical methods: H2SO4 2%, 160 °C; NaOH 2%, 140 °C; NaOCl 2%, 140 °C; autohydrolysis: H2O, 190 °C. Subsequently, BioH2 production was carried out using the pretreated material with the highest digestibility applying a Taguchi experimental array to identify the optimal operating conditions. The results showed a higher glucose released from pretreated corncob with H2SO4 (134.7 g/L) compared to pretreated materials by autohydrolysis, NaOH and NaOCl (123 g/L, 89.8 g/L and 52.9 g/L, respectively). The mixed culture was able to hydrolyze the pretreated corncob and produce 575 mL of H2 (at 35 °C, pH 5.5, 1:2 ratio of BRF:Clac and 5% of solids loading) equivalent to 132 L H2/Kg of biomass.