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Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog

2021; Elsevier BV; Volume: 53; Linguagem: Inglês

10.1016/j.scr.2021.102375

1876-7753

Sho Yoshimatsu, Kazuya EDAMURA, Yumi Yoshii, Aozora Iguchi, Hirotaka Kondo, Hisashi Shibuya, Tsukika Sato, Seiji Shiozawa, Hideyuki Okano,

Animal Genetics and Reproduction

We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, which showed standard characteristics of pluripotency such as a strong gene expression profile of pluripotency markers, differentiation potential into all three germ layers, and normal karyotype (78XY). Furthermore, we demonstrated targeted integration of 2A-EGFP into the canine NANOS3 locus. The novel iPSC line would be a useful resource for stem cell research and regenerative veterinary medicine.