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Chromolaena laevigata (Asteraceae) as a source of endophytic non-aflatoxigenic Aspergillus flavus: chemical profile in different culture conditions and biological applications

2021; Springer Nature; Volume: 52; Issue: 3 Linguagem: Inglês

10.1007/s42770-021-00502-6

1678-4405

Rodolfo Bento Balbinot, Josiane A. Monteiro de Oliveira, Darlon I. Bernardi, Andressa Domingos Polli, Júlio César Polonio, Márcia Regina Pereira Cabral, Érica Benassi Zanqueta, Eliana Harue Endo, Jean Eduardo Meneguello, Rosilene Fressatti Cardoso, João Lúcio de Azevedo, Benedito Prado Dias Filho, Tânia Ueda‐Nakamura, Marta Regina Barrotto do Carmo, María Helena Sarragiotto, João Alencar Pamphile, Débora Cristina Baldoqui,

Microbial Natural Products and Biosynthesis

Endophytes are microorganisms that form symbiotic relationships with their host. These microorganisms can produce a variety of secondary metabolites, some of which have inhibitory effects on pests and pathogens or even act to promote plant growth. Due to these characteristics, these microorganisms are used as sources of biologically active substances for a wide range of biotechnological applications. Based on that, the aim of this study was to evaluate the production of metabolites of the endophytic Aspergillus flavus CL7 isolated from Chromolaena laevigata, in four different cultivation conditions, and to determine the antimicrobial, cytotoxic, antiviral, and antioxidant potential of these extracts. The multiphasic approach used to identify this strain was based on morphology and ITS gene sequence analysis. The chemical investigation of A. flavus using potato dextrose and minimal medium, using both stationary and agitated methods, resulted in the isolation of kojic acid, α-cyclopiazonic acid, and 20,25-dihydroxyaflavinine. Another 18 compounds in these extracts were identified by UHPLC-HRMS/MS, of which dideacetyl parasiticolide A has been described for the first time from A. flavus. Aflatoxins, important chemomarkers of A. flavus, were not detected in any of the extracts, thus indicating that the CL7 strain is non-aflatoxigenic. The biological potential of all extracts was evaluated, and the best results were observed for the extract obtained using minimal medium against Trichophyton rubrum and Mycobacterium tuberculosis.