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CRISPR-Mediated Strand Displacement Logic Circuits with Toehold-Free DNA

2021; American Chemical Society; Volume: 10; Issue: 5 Linguagem: Inglês

10.1021/acssynbio.0c00649

2161-5063

Roser Montagud‐Martínez, María Heras‐Hernández, Lucas Goiriz, José‐Antonio Daròs, Guillermo Rodrigo,

Advanced Memory and Neural Computing

DNA nanotechnology, and DNA computing in particular, has grown extensively over the past decade to end with a variety of functional stable structures and dynamic circuits. However, the use as designer elements of regular DNA pieces, perfectly complementary double strands, has remained elusive. Here, we report the exploitation of CRISPR-Cas systems to engineer logic circuits based on isothermal strand displacement that perform with toehold-free double-stranded DNA. We designed and implemented molecular converters for signal detection and amplification, showing good interoperability between enzymatic and nonenzymatic processes. Overall, these results contribute to enlarge the repertoire of substrates and reactions (hardware) for DNA computing.