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Comprehensive analysis of the R2R3-MYB transcription factor gene family in Populus trichocarpa

2021; Elsevier BV; Volume: 168; Linguagem: Inglês

10.1016/j.indcrop.2021.113614

1872-633X

Xiaoyu Yang, Juan Li, Ting Guo, Bin Guo, Zhong Chen, Xinmin An,

Plant biochemistry and biosynthesis

• Systematic genome-wide analysis of R2R3-MYB gene family were conducted in Populus . • PtrMYB transcription factors play crucial roles in development and stress responses in Populus . • This work will contribute to functional characterization of PtrMYB genes and breeding improvements in Populus . The MYB proteins constitute one of the most important transcription factor families in plants, and are involved in various plant-specific processes, such as development, metabolism, and stress responses. Populus is a major industrial tree species for building, papermaking, furniture, and biofuels. This study presents a systematic genome-wide analysis of the MYB family in Populus . In total, 354 genes were identified as candidate MYB genes and divided into four subfamilies, of which 196 R2R3-MYBs were then investigated further. These Populus R2R3-MYBs were classified into 45 subgroups based on comparative phylogenetic analysis with Arabidopsis thaliana , which were solidly backed by highly conserved intron/exon structures and motifs outside the MYB domain. Many types of cis-regulatory elements were detected in the promoter region, mainly related to development, light response, phytohormone response, and environmental stress response, directly reflecting the functional diversification of Populus R2R3-MYB members. Collinearity analysis revealed that purifying selection became a primary driving force during R2R3-MYB gene evolution, and that segmental and tandem duplications played critical roles in the expansion of this family in Populus . Expression profiles from publicly available RNA-Seq data and real-time quantitative PCR analysis displayed distinct expression patterns of PtrMYB genes among tissues and organs, as well as in response to drought stress. Overall, this work establishes a solid foundation for further functional dissection of MYB gene families, as well as breeding improvement for stress resistance and wood properties in Populus.