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Efficacy of Terminalia catappa-AgNP nanocomposite towards Saprolegnia parasitica infection in angelfish (Pterophyllum scalare) eggs

2021; Elsevier BV; Volume: 543; Linguagem: Inglês

10.1016/j.aquaculture.2021.736914

1873-5622

Juliana Oliveira Meneses, Izadora Cibely Alves da Silva, Ana Flávia Santos da Cunha, João Carlos Nunes de Souza, Victor Ruan Silva Nascimento, Cindy Caroline Moura Santos, Fernanda dos Santos Cunha, Peterson Emmanuel Guimarães Paixão, Joel Artur Rodrigues Dias, Natalino da Costa Sousa, Márcia Valéria Silva do Couto, Higo Andrade Abe, Ricardo Marques Nogueira Filho, Thays Brito Reis Santos, Francisco José dos Santos, Luiz Pereira da Costa, Juliana Cordeiro Cardoso, Rodrigo Yudi Fujimoto,

Advanced Nanomaterials in Catalysis

A nanocomposite based on silver nanoparticles (AgNPs) and extract of Terminalia catappa was developed, characterized, and evaluated in in vitro and in vivo conditions against Saprolegnia parasitica infection. The nanocomposite contained spheroidal silver-nanoparticles (1–55 nm) and presented active compounds as gallic acid, ellagic acid, and α and β punicalagin. The nanoparticles remained stable for one year after its production. A synergistic effect was observed between AgNPs and extract under in vitro and in vivo conditions against different stages of fungal development. In an in vitro assay, the nanocomposite showed fungistatic and fungicide effects to the fungal mycelium in solid and liquid media, respectively, through an increase in the contact surface. In an in vivo bioassay, the lowest concentration of nanocomposite (T1 = 45.75 μg.L−1 AgNPs +62.5 μg.L−1 T. catappa extract) demonstrated similar efficiency as the positive control (methylene blue) in preventing zoospore infectivity in eggs of angelfish (Pterophyllum scalare). The fungal zoospores were more sensitive to the nanocomposite than fungal mycelia. Our results exhibited the use of a nanocomposite containing AgNPs and T. catappa aqueous extract could reduce the required effective concentrations of AgNPs against saprolegniosis in fish eggs, thus, it may as an alternative to improve fish larval survival at the hatchery stage.