Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle

2021; Multidisciplinary Digital Publishing Institute; Volume: 12; Issue: 6 Linguagem: Inglês

10.3390/genes12060806

2073-4425

Thaís Miranda Silva Freitas, Juliana Moraes Dias, Luanna Guimarães, Sáudio Vieira Peixoto, Rayanne Henrique Santana da Silva, Kareem Rady Badr, Maria Ivete de Moura, Adriana Santana do Carmo, Vincenzo Landi, Maria Clorínda Soares Fíoravantí,

Cancer-related molecular mechanisms research

Susceptibility to diseases is inherited and can be transmitted between populations. Single-nucleotide polymorphism (SNPs) in genes related to immune response is associated with diseases in cattle. This study investigated SNPs in the genomic region of cytokines in 702 samples of Curraleiro Pé-Duro cattle and associated them with the occurrence of antibodies in brucellosis, leptospirosis, neosporosis, leukosis, infectious bovine rhinotracheitis (IBR), and bovine viral diarrhea (BVD) tests. DNA samples were evaluated by the kompetitive allele-specific polymerase chain reaction (KASP) method to identify polymorphisms. The gametic phase and SNP haplotypes were determined with the help of PHASE 2.1.1 software. Haplotypes were associated with serological results against Brucella abortus, Leptospira sp., Neospora caninum, leukosis, infectious rhinotracheitis, and BVD using univariate analysis followed by logistic regression. Haplotype 2 of TLR2 was present in 70% of the animals that tested positive for N. caninum infection. Haplotypes of TLR10 and TLR6 and IL10RA were more common in seronegative animals. Haplotypes related to the gene IL10RA were associated with animals negative to all infections. Curraleiro Pé-Duro cattle presented polymorphisms related to resistance to bacterial, viral, and N. caninum infections.