Droplet Microfluidic Optimisation Using Micropipette Characterisation of Bio-Instructive Polymeric Surfactants
2021; Multidisciplinary Digital Publishing Institute; Volume: 26; Issue: 11 Linguagem: Inglês
10.3390/molecules26113302
ISSN1433-1373
AutoresCharlotte A. Henshaw, Adam A. Dundas, Valentina Cuzzucoli Crucitti, Morgan R. Alexander, Ricky Wildman, Felicity R. A. J. Rose, Derek J. Irvine, Philip M. Williams,
Tópico(s)Electrowetting and Microfluidic Technologies
ResumoDroplet microfluidics can produce highly tailored microparticles whilst retaining monodispersity. However, these systems often require lengthy optimisation, commonly based on a trial-and-error approach, particularly when using bio-instructive, polymeric surfactants. Here, micropipette manipulation methods were used to optimise the concentration of bespoke polymeric surfactants to produce biodegradable (poly(d,l-lactic acid) (PDLLA)) microparticles with unique, bio-instructive surface chemistries. The effect of these three-dimensional surfactants on the interfacial tension of the system was analysed. It was determined that to provide adequate stabilisation, a low level (0.1% (w/v)) of poly(vinyl acetate-co-alcohol) (PVA) was required. Optimisation of the PVA concentration was informed by micropipette manipulation. As a result, successful, monodisperse particles were produced that maintained the desired bio-instructive surface chemistry.
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