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Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression

2021; Elsevier BV; Volume: 183; Linguagem: Inglês

10.1016/j.ijbiomac.2021.05.120

1879-0003

Wan-Rong Bao, Quanwei Zhang, Hongming Zheng, Lifeng Li, Man Liu, Huiyuan Cheng, Tin-Long Wong, Ge Zhang, Aiping Lü, Zhaoxiang Bian, Dik‐Lung Ma, Chung‐Hang Leung, Quan‐Bin Han,

Cancer Cells and Metastasis

Radix Astragali polysaccharide RAP has been reported to play a crucial role in hematopoiesis without a clear mechanism. In this study, RAP's effects to enhance the recovery of cyclophosphamide (Cy)-suppressed bone marrow and blood cells is confirmed in vivo first. Confocal micrographs demonstrated the interesting direct binding of FITC-RAP to hematopoietic stem cells (HSC) in bone marrow. RAP protects both mice and human HSC in terms of cell morphology, proliferation, and apoptosis. RNA-sequencing and shRNA approaches revealed FOS to be a key regulator in RAP's protection. These evidences provide an unreported mechanism that RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression.