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Thiol/disulfide homeostasis impaired in patients with primary Sjögren's syndrome

2021; De Gruyter; Volume: 40; Issue: 3 Linguagem: Inglês

10.5937/jomb0-27281

1452-8258

Cakmak Yılmaz, Emin Gemcioğlu, Salih Başer, Şükran Erten, Özcan Erel,

Endoplasmic Reticulum Stress and Disease

Primary Sjögren's syndrome (pSS) is a disease associated with the overexpression of proinflammatory cytokines, and oxidative stress is one of the factors responsible for its etiopathogenesis. This study aimed to investigate the thiol/disulphide homeostasis in pSS patients.The study included 68 pSS patients and 69 healthy controls. Thiol/disulphide homeostasis (total thiol, native thiol, and disulphide levels) was measured using the automatic spectrophotometric method developed by Erel and Neselioglu, and the results of the 2 groups were compared.The gender and age distributions of the pSS and control groups were similar (P = 0.988 and P = 0.065). Total thiol and native thiol levels were lower in the pSS group than in the control group (470.08 ± 33.65 µmol/L vs. 528.21 ± 44.99 µmol/L, P < 0.001, and 439.14 ± 30.67 µmol/L vs. 497.56 ± 46.70 µmol/L, P < 0.001, respectively). There were no differences in disulphide levels between groups [17.00 (range 0.70-217.0) µmol/L vs. 14.95 (range 2.10-40.10) µmol/L, P = 0.195].It was concluded that the thiol/disulphide balance shifted towards disulphide in patients with pSS.Primarni Sjögrenov sindrom (pSS) je bolest povezana sa prekomernom ekspresijom proinflamatornih citokina, dok je oksidativni stres jedan od faktora odgovornih za njegovu etiopatogenezu. Cilj ove studije je bio da ispita tioldisulfidnu homeostazu kod pacijenata sa pSS-om.Studija je obuhvatila 68 pacijenata sa pSS-om i 69 zdravih kontrolnih pojedinaca. Homeostaza tiol-disulfida (nivoi ukupnog tiola, nativnog tiola i disulfida) je izmerena pomoću automatske spektrofotometrijske metode koju su razvili Erel i Neselioglu. Rezultati dve grupe su upoređeni.Raspodela po polu i starosti pacijenata sa pSSom i pojedinaca u kontrolnoj grupi je bila slična (P = 0,988 i P = 0,065). Ukupni nivoi tiola i nativnog tiola bili su niži u grupi sa pSS-om nego u kontrolnoj grupi (470,08 ± 33,65 µmol/L naspram 528,21 ± 44,99 µmol/L, P <0,001 i 439,14 ± 30,67 µmol/L naspram 497,56 ± 46,70 µmol/L, P <0,001, respektivno). Nije bilo razlika u nivoima disulfida između grupa [17,00 (opseg 0,70-217,0) µmol/L naspram 14,95 (opseg 2,10-40,10) µmol/L, P = 0,195].Zaključeno je da se ravnoteža tiol/disulfid pomera prema disulfidu kod pacijenata sa pSS-om.