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Redox feedback regulation of ANAC089 signaling alters seed germination and stress response

2021; Cell Press; Volume: 35; Issue: 11 Linguagem: Inglês

10.1016/j.celrep.2021.109263

2639-1856

Pablo Albertos, Kiyoshi Tatematsu, Isabel Mateos, Inmaculada Sánchez-Vicente, Alejandro Fernández-Arbaizar, Kazumi Nakabayashi, Eiji Nambara, Marta Godoy, José M. Franco, Roberto Solano, Davide Gerna, Thomas Roach, Wolfgang Stöggl, Ilse Kranner, Carlos Perea-Resa, Julio Salinas, Óscar Lorenzo,

Legume Nitrogen Fixing Symbiosis

The interplay between the phytohormone abscisic acid (ABA) and the gasotransmitter nitric oxide (NO) regulates seed germination and post-germinative seedling growth. We show that GAP1 (germination in ABA and cPTIO 1) encodes the transcription factor ANAC089 with a critical membrane-bound domain and extranuclear localization. ANAC089 mutants lacking the membrane-tethered domain display insensitivity to ABA, salt, and osmotic and cold stresses, revealing a repressor function. Whole-genome transcriptional profiling and DNA-binding specificity reveals that ANAC089 regulates ABA- and redox-related genes. ANAC089 truncated mutants exhibit higher NO and lower ROS and ABA endogenous levels, alongside an altered thiol and disulfide homeostasis. Consistently, translocation of ANAC089 to the nucleus is directed by changes in cellular redox status after treatments with NO scavengers and redox-related compounds. Our results reveal ANAC089 to be a master regulator modulating redox homeostasis and NO levels, able to repress ABA synthesis and signaling during Arabidopsis seed germination and abiotic stress.