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Tergitol-15-S-9 Inactivates SARS-CoV-2 and Boosts Immunoassay Signals

2021; Future Science Ltd; Volume: 71; Issue: 1 Linguagem: Inglês

10.2144/btn-2021-0044

1940-9818

Michael G. Berg, Eitan Israeli, Erin Quaco, Gavin Cloherty, Philip M. Hemken,

SARS-CoV-2 and COVID-19 Research

Inactivation of SARS-CoV-2 virus is necessary to mitigate risk but may interfere with diagnostic assay performance. We examined the effect of heat inactivation on a prototype SARS-CoV-2 antigen immunoassay run on the ARCHITECT automated analyzer. Recombinant full-length SARS-CoV-2 nucleocapsid protein and virus lysate detection was reduced by 66 and 31%, respectively. Several nonionic detergents were assessed as inactivation alternatives based on infectivity in cultured Vero CCL81 cells. Incubation of SARS-CoV-2 in 0.1% Tergitol 15-S-9 for 10 min significantly reduced infectivity and increased the immunoassay signal for cultured lysate and patient specimens. Tergitol 15-S-9 can inactivate SARS-CoV-2 while preserving epitopes on the nucleocapsid protein for enhanced detection by immunoassay antibodies.