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Mycoplasma spp. em cobras de cativeiro (Boa constrictor e Bothrops atrox) do Brasil

2021; UNIVERSIDADE FEDERAL DE SANTA MARIA; Volume: 51; Issue: 1 Linguagem: Português

1678-4596

Bárbara Souza Neil Magalhães, Leandro dos Santos Machado, Arthur de Almeida Figueira, Thomas Salles Dias, Thiago de Almeida Feijó, Maria Lúcia Barreto, Giulia de Almeida Tuffanelli, Nathalie Costa da Cunha, Elmiro Rosendo do Nascimento, Virgínia Léo de Almeida Pereira, Nádia Regina Pereira Almosny,

Turtle Biology and Conservation

EnglishAlthough rare, mycoplasmas are included among the causes of respiratory diseases in reptiles and, in the order Squamata, three reports of these microorganisms causing diseases in pythons have already been reported. This study aimed to evaluate the occurrence of Mycoplasma species in captive snakes. A total of 26 snakes of the families Pythonidae (13), Boidae (7), Viperidae (5) and Colubridae (1) from RioZoo, Brazil, were evaluated. Animals were examined to determine clinical signs consistent with any infectious disease. Tracheal swab samples from snakes were collected in Frey medium and analyzed for the presence of Mycoplasma spp.by isolation and a genus-specific PCR. DNA sequencing analyses of six positive samples by PCR were carried out to identify the species. Using isolation 19.23% (5/26) was positive, while 65.38% (17/26) of the animals were positive by PCR. Based on the analyses of the six sequences obtained, there was similarity with a Mycoplasma spp. previously described in a phyton and, M. agassizii and M. testudineum reported in chelonians. This is the first report of Mycoplasma spp. in animals of the families Boidae and Viperidae. Mycoplasma spp. were detected in snakes with and without clinical signs. The mycoplasmas reported resented identity (range, 95% to 100%) to others already described in reptiles. There was no relationship between the presence of Mycoplasma spp. and clinical signs portuguesEmbora raros, os micoplasmas estao incluidos entre as causas de doencas respiratorias em repteis e, na ordem Squamata, ja foram realizados tres relatos destes microrganismos causando doenca em pitons. Este estudo teve como objetivo avaliar a ocorrencia de especies de Mycoplasma em serpentes em cativeiro. Foram avaliadas 26 serpentes das familias Pythonidae (13), Boidae (7), Viperidae (5) e Colubridae (1) do RioZoo, Brasil. Os animais foram examinados para determinar sinais clinicos consistentes com qualquer doenca infecciosa. Amostras de swab traqueal de cobras foram coletadas em meio Frey e analisadas por isolamento microbiologico e pela tecnica da PCR para identificar Mycoplasma spp. As amostras positivas para o genero Mycoplasma spp. foram submetidas ao sequenciamento genetico para identificacao das especies. No isolamento, 19,23% (5/26) foram positivos, enquanto 65,38% (17/26) dos animais foram positivos por PCR. Com base nas analises das seis sequencias obtidas, houve similaridade com o Mycoplasma spp. descrito anteriormente em um piton e M. agassizii e M. testudineum encontrados em quelonios. Este e o primeiro relato de Mycoplasma spp. em animais das familias Boidae e Viperidae. Mycoplasma spp. foi detectado em serpentes com e sem sinais clinicos. Os micoplasmas encontrados apresentaram semelhanca genetica com outros ja descritos em repteis. Nao houve relacao entre a presenca de Mycoplasma spp. e sinais clinicos