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A fast and efficient preparative method for separation and purification of main bioactive xanthones from the waste of Garcinia mangostana L. by high-speed countercurrent chromatography

2021; Elsevier BV; Volume: 14; Issue: 8 Linguagem: Inglês

10.1016/j.arabjc.2021.103252

1878-5379

Ricardo Felipe Alexandre de Mello, Wandson Braamcamp de Souza Pinheiro, Jaisielle Kelem França Benjamim, Francilia Campos de Siqueira, Renan Campos Chisté, Alberdan Silva Santos,

Phytochemistry and biological activity of medicinal plants

In this work, we present a preparative elution method by high-speed countercurrent chromatography (Prep-HSCCC) for the separation and isolation of the main bioactive xanthones present in the alcoholic extract of Garcinia mangostana L. agro-industrial waste. The method proved to be quite efficient, operating with a two-phase solvent system consisting of Methanol/water/Ethanol/Hexane/methyl tert-butyl ether (6:3:1:6:4v/v), tail-head elution mode, with mobile phase flow of 5 mL/min and rotation speed of 800 rpm. The method demonstrated repeatability (RSD = 3.8–6.5%) and allowed the isolation of α-mangostin and γ-mangostin xanthones, both with purities above 93%, in a single step, performed in a time of 35 min. Isolated substances were identified by HPLC-PDA-MS and 1H and 13C NMR spectroscopy. Both xanthones were efficient scavengers of DPPH· and ABTS+ radicals in a concentration-dependent manner with EC50 at 8.43 and 3.31 µg/mL, respectively, for γ-mangostin, and 71.10 and 29.33 µg/mL, respectively, for α-mangostin.