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Impaired mammalian sperm function and lower phosphorylation signaling caused by the herbicide Roundup® Ultra Plus are due to its surfactant component

2021; Elsevier BV; Volume: 172; Linguagem: Inglês

10.1016/j.theriogenology.2021.05.026

1879-3231

Mercedes Torres-Badia, Soraya Solar-Málaga, David Martín‐Hidalgo, Ana Hurtado de Llera, Andrea Gomez-Candelo, Luis García, Lauro González‐Fernández, Marı́a J. Bragado,

Pesticide Exposure and Toxicity

The use of worldwide glyphosate-based herbicide Roundup® is growing and to date its effects on mammalian spermatozoa are controversial. This study aims to investigate the functional impact of in vitro exposure of pig spermatozoa to low concentrations of Roundup® Ultra Plus (RUP), similar to those present as environment contaminants, to its active ingredient glyphosate , and to the non-active component, surfactant polyoxyethyleneamine (POEA). Pig spermatozoa were incubated in Tyrode's basal medium (TBM) or Tyrode's complete medium (TCM) (1 h at 38.5 °C) with several RUP dilutions or equivalent concentrations of glyphosate or POEA. RUP treatment causes a significant dilution-dependent decrease in sperm motility , a significant increase in plasma membrane disorganization and reduction in GSK3β phosphorylation (TBM) and in two PKA substrates (TBM and TCM), whereas does not affect sperm viability or mitochondrial membrane potential (MMP). Equivalent glyphosate concentrations do not affect any functional sperm parameters. However, POEA concentrations equivalent to RUP dilutions mimic all RUP sperm effects: decrease sperm motility in a concentration-dependent manner, increase sperm plasma membrane lipid disorder and significantly inhibit GSK3β phosphorylation (TBM) and two PKA substrates without affecting sperm viability or MMP. In summary, low concentrations RUP herbicide cause sperm motility impairment without affecting sperm viability. This adverse effect could be likely due to a detrimental effect in the plasma membrane lipid organization and to inhibition of phosphorylation of both, GSK3β and specific PKA substrates. Importantly, our results indicate that negative effects of low RUP concentrations in pig spermatozoa function are likely caused by the surfactant included in its formulation and no by its active ingredient glyphosate. • Low concentrations of the herbicide Roundup impair pig sperm motility without affecting sperm viability. • Roundup adverse effects are caused by the surfactant included in its formulation but no by its active ingredient glyphosate. • Roundup adverse effects are likely due to a detrimental effect of surfactant at the plasma membrane integrity. • Roundup negative effectsinvolve the inhibition of phosphorylation pathways that control sperm motility: GSK3α/β and PKA.