First Report of Fusarium sacchari Causing Sugarcane Wilt in Bangladesh
2021; American Phytopathological Society; Volume: 106; Issue: 1 Linguagem: Inglês
10.1094/pdis-04-21-0681-pdn
ISSN1943-7692
AutoresSwapan Kumar Paul, Nur Uddin Mahmud, Dipali Rani Gupta, Mohammad Nur Alam, Moutoshi Chakraborty, Tofazzal Islam,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 106, No. 1First Report of Fusarium sacchari Causing Sugarcane Wilt in Bangladesh PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Fusarium sacchari Causing Sugarcane Wilt in BangladeshS. K. Paul, N. U. Mahmud, D. R. Gupta, M. N. Alam, M. Chakraborty, and M. T. IslamS. K. PaulDepartment of Agronomy, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh, N. U. MahmudInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh, D. R. GuptaInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh, M. N. AlamBangladesh Sugarcrop Research Institute, Ishurdi 6620, Pabna, Bangladesh, M. Chakrabortyhttps://orcid.org/0000-0002-2699-9284Institute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh, and M. T. Islam†Corresponding author: M. T. Islam; E-mail Address: [email protected]https://orcid.org/0000-0002-7613-0261Institute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshAffiliationsAuthors and Affiliations S. K. Paul1 N. U. Mahmud2 D. R. Gupta2 M. N. Alam3 M. Chakraborty2 M. T. Islam2 † 1Department of Agronomy, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh 2Institute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh 3Bangladesh Sugarcrop Research Institute, Ishurdi 6620, Pabna, Bangladesh Published Online:8 Jan 2022https://doi.org/10.1094/PDIS-04-21-0681-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleWilt is one of the destructive fungal diseases of sugarcane (Saccharum officinarum L.) affecting production in many sugarcane growing regions including Bangladesh. In July 2018, sugarcane plants showing wilting symptoms including withered, dried leaves and shriveling stalks with brown to pinkish discoloration in internal tissues (Viswanathan 2013) were collected from sugarcane fields in the Gazipur district (23°54′41″ N, 90°23′20″ E) of Bangladesh. The average disease incidence was 17% in 60 ha of sugarcane fields that were sampled for diseased plants with visible symptoms. Ten infected stalks (30 cm) were collected from five severely infected fields. The stalks were surface sterilized with 70% ethanol for 1 min followed by 0.2% sodium hypochlorite for 3 min and then rinsed three times with distilled water. Small pieces (2 mm × 2 mm) of the inner discolored tissue were cut with a sterile scalpel and placed on half strength potato dextrose agar (PDA) medium followed by incubation for 7 days at 25°C. A total of 10 isolates were recovered from the infected sugarcane stalks and were purified by the single spore culture method. Isolates produced floccose and cottony colonies with white mycelia and dark violet pigmentation on the lower surface of the PDA plate. Microconidia were oval, generally, without septations, measuring 5 to 9.6 µm long and 1.8 to 3.7 µm wide. Macroconidia were not found on PDA. Conidiophores produced mono- or polyphialides and conidia were arranged in false heads on conidiophores, which is consistent with Fusarium sacchari (Duan et al. 2019). For molecular identification, DNA was extracted from two representative isolate BTFSS1 and BTFSS6. The internal transcribed spacer (ITS) region, translation elongation factor 1-α (TEF-1α) gene, and RNA polymerase II (RPB2) genes were amplified using primers ITS1/ITS4, EF1α-F/EF1α-R, and 5F2/11aR, respectively. Obtained ITS sequences (GenBank accession nos. MT176492.1 and MT177209.1) showed 99.8% similarity with the sequences from F. sacchari strain olowEKT1 (MK072727.1). The sequence identity of TEF-1α (MW558264.1 and MW507839.1) was 99.36% with the sequence of F. sacchari strain FF031 (MK152501.1), and RPB2 (MW558265.1 and MW507840.1) was 99.42% with the sequence of F. sacchari strain FF001 (MK152508.1), respectively. The isolates BTFSS1 and BTFSS6 were identified as F. sacchari based on the sequence alignment of ITS, TEF-1α, and RPB2 and on molecular phylogenetic analyses by the maximum likelihood method. To confirm the pathogen as a causal agent of sugarcane wilt, pathogenicity tests were conducted by the plug method (Viswanathan et al. 2011). Ten plants (cv. BSRI Akh 42) were inoculated with 1 × 105 conidia ml−1 conidial suspension of the isolate BTFSS1. A borehole (5-mm-diameter) was made on the third or fourth internode from the base of the sugarcane plant in which the conidial suspension was placed and the bore place was sealed with cane tissues. Control plants were inoculated with deionized distilled water. All canes were cut longitudinally to evaluate the symptoms at 15 and 30 days after inoculation. Inoculated stalks exhibited tissue discoloration similar to those observed in infected sugarcane fields, whereas the control remained nonsymptomatic. Pathogens were reisolated from the artificially inoculated cane and confirmed by morphological and molecular tests. Sugarcane is the second important cash crop and the only source of white sugar in Bangladesh (Rahman et al. 2016). This is the first confirmed report that F. sacchari is the causal pathogen of sugarcane wilt in Bangladesh.The author(s) declare no conflict of interest.References:Duan, C. X., et al. 2019. Plant Dis. 103:2674. https://doi.org/10.1094/PDIS-04-19-0829-PDN Link, ISI, Google ScholarRahman, M. S., et al. 2016. Sugar Tech 18:627. https://doi.org/10.1007/s12355-016-0489-z Crossref, ISI, Google ScholarViswanathan, R. 2013. J. Sugar Res. 3:86. Google ScholarViswanathan, R. M., et al. 2011. Sugar Tech 13:68. https://doi.org/10.1007/s12355-011-0066-4 Crossref, ISI, Google ScholarS. K. Paul and N. U. Mahmud contributed equally.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 1 January 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms of Macrophomina phaseolina in melon (R. Cohen et al.). Photo credit: R. Cohen. Jute plant infected with papaya ring spot virus (PRSV) (sample MG16-004) (C. Biswas et al.). Photo credit: V. Ramesh Babu. Metrics Article History Issue Date: 7 Feb 2022 Published: 8 Jan 2022 First Look: 22 Jun 2021 Accepted: 20 Jun 2021 Page: 319 Information© 2022 The American Phytopathological SocietyKeywordssugarcane wiltFusariumconidiaThe author(s) declare no conflict of interest.PDF downloadCited byFirst report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh15 February 2023 | F1000Research, Vol. 9
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