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IMMUNOHISTOCHEMICAL AND HISTOPATHOLOGICAL STUDY FOR THE DIAGNOSIS AND CLASSIFICATION OF LYMPHOMAS

2021; Wiley; Volume: 39; Issue: S2 Linguagem: Inglês

10.1002/hon.15_2881

1099-1069

Julio Jiménez Galainena, J. L. Jimenez-Hoyuela Garcia, Zailí Gutiérrez Aleaga, Margarita Amigó de Quesada, M. Nazario Dolz, L Heredia Manzano, Elena Pérez, W. Marcial Martínez, A. Columbié Molina, Elías Gracia Medina, Tamara González Madruga, Jennys Peraza Bordao,

Oral Health Pathology and Treatment

Introduction: The histopathological classification and subclassification of lymphomas in hematopathology is the gold standard for the diagnosis of these diseases. Different techniques, such as immunohistochemistry, fluorescent in situ hybridization and molecular studies have been included in the diagnostic field of Anatomic Pathology. The information obtained with these techniques combined with conventional light microscopy study allows a specific morphological diagnosis according to the WHO Classification permitting the best treatment in the patients. Objective: The study with immunohistochemical techniques was to classify and subclassify these neoplastic lymphoid processes. Material and methods: The study included 2053 biopsies of different sites diagnosed as lymphomas at the Pathology Department of the National Oncology Institute, Havana City, Cuba from the period of 2007 to 2019 with a panel of different antibodies for hematolymphoid processes employing the technique of immunohistochemistry in paraffin blocks. The manual and automated immunohistochemical staining were used. The visualization systems included the Polymer Detection System and the Ultraview Universal DAB Detection Kit. Results: The Non-Hodgkin´s B Cell Lymphomas were the predominant immunophenotypic type (1478 cases)(71.9%).The B Cell Lymphomas CD20 positive were 1311 cases (63.8 %).The Diffuse Large B Cell Lymphomas were the most frequent histopathological type (725 cases)(35.3%).The Diffuse Large B Cell Lymphoma molecular subtypes classification (Hans Algorithm)(CD10/Bcl6/Mum1) by immunohistochemistry were the following in the cases studied (96 cases)(2015 -2019): GBC (29 cases)(30.2%), ABC (45 cases)(46.8%),non GBC / non ABC (18 cases)(18.7%), Double expressor (4 cases)(4.16%).The Peripheral T Cell Lymphomas (81 cases) and Anaplastic Large Cell Lymphoma(67 cases) were the principal histologies diagnosed in the group of Non-Hodgkin´s T Cell Lymphomas (153 cases)(7.45%).The extranodal sites were 941 cases (45.8%) and the Head and Neck location was the common site diagnosed in this group (195 cases)(20.7%). Conclusion: The study showed the different frequencies, locations and histological types of the lymphomas in our daily work of a period of time of 13 years in the Immunohistochemistry Laboratory at the Pathology Department. Keywords: GBC (Germinal center B-cell-like), ABC (Activated B-cell-like), non GBC / non ABC (non Germinal center B-cell-like) / (non Activated B-cell-like) Keywords: Pathology and Classification of Lymphomas No conflicts of interests pertinent to the abstract.