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Association between cycle threshold ( C t ) values and clinical and laboratory data in inpatients with COVID‐19 and asymptomatic health workers

2021; Wiley; Volume: 93; Issue: 10 Linguagem: Inglês

10.1002/jmv.27170

1096-9071

Juan Pablo Ramirez‐Hinojosa, Yunuen Rodríguez-Sánchez, Angel Kaleb Romero‐Gonzalez, Marisol Chavez‐Gutierrez, Nelly Raquel Gonzalez‐Arenas, Aurora Ibarra‐Arce, Sara Arroyo‐Escalante, Beatríz Zavaleta‐Villa, Moisés León‐Juárez, Víctor Javier Cruz-Holguín, Luz Elena Espinosa de los Monteros-Pérez, Angélica Olivo-Dı́az, Rigoberto Hernández‐Castro, Lourdes Suarez‐Roa, Héctor Prado, Octavio Sierra-Martínez, Guillermina Ávila, Ana Flisser, Pablo Maravilla, Mirza Romero‐Valdovinos,

Biosensors and Analytical Detection

In-house assays for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by quantitative reverse-transcription polymerase chain reaction (qRT-PCR), are feasible alternatives, particularly in developing countries. Cycle threshold (Ct ) values obtained by qRT-PCR were compared with clinical and laboratory data from saliva of inpatients with COVID-19 and asymptomatic health workers (AHW) were studied. Saliva specimens from 58 inpatients confirmed by qRT-PCR for SARS-CoV-2 using nasopharyngeal specimens, and 105 AHW were studied by qRT-PCR using three sets of primers for the N (N1, N2, and N3) gene of SARS-CoV-2, according to the CDC Diagnostic Panel protocol, showing a positivity of 88% for inpatients and 8% for AHW. Bivariate analysis revealed an association between Ct < 38.0 values for N2 and mechanical ventilation assistance among patients (p = .013). In addition, values of aspartate-transaminase, lactate dehydrogenase, and ferritin showed significant correlations with Ct values of N1 and N3 genes in inpatients. Therefore, our results show that Ct values correlate with some relevant clinical data for inpatients with COVID-19.