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The cellular architecture of the antimicrobial response network in human leprosy granulomas

2021; Nature Portfolio; Volume: 22; Issue: 7 Linguagem: Inglês

10.1038/s41590-021-00956-8

1529-2916

Feiyang Ma, Travis K. Hughes, Rosane M. B. Teles, Priscila Ribeiro Andrade, Bruno Jorge de Andrade Silva, Olesya Plazyo, Lam C. Tsoi, Tran Do, Marc H. Wadsworth, Aislyn Oulee, Marîa Teresa Ochoa, Euzenir Nunes Sarno, M. Luisa Iruela‐Arispe, Eynav Klechevsky, Bryan D. Bryson, Alex K. Shalek, Barry R. Bloom, Jóhann E. Guðjónsson, Matteo Pellegrini, Robert L. Modlin,

Immunotherapy and Immune Responses

Granulomas are complex cellular structures composed predominantly of macrophages and lymphocytes that function to contain and kill invading pathogens. Here, we investigated the single-cell phenotypes associated with antimicrobial responses in human leprosy granulomas by applying single-cell and spatial sequencing to leprosy biopsy specimens. We focused on reversal reactions (RRs), a dynamic process whereby some patients with disseminated lepromatous leprosy (L-lep) transition toward self-limiting tuberculoid leprosy (T-lep), mounting effective antimicrobial responses. We identified a set of genes encoding proteins involved in antimicrobial responses that are differentially expressed in RR versus L-lep lesions and regulated by interferon-γ and interleukin-1β. By integrating the spatial coordinates of the key cell types and antimicrobial gene expression in RR and T-lep lesions, we constructed a map revealing the organized architecture of granulomas depicting compositional and functional layers by which macrophages, T cells, keratinocytes and fibroblasts can each contribute to the antimicrobial response. Modlin and colleagues examined the skin lesions of human leprosy patients using single-cell RNA sequencing coupled to cellular spatial mapping. Their analysis maps the architecture of granulomas in leprosy lesions from patients with leprosy with localized disease (tuberculoid leprosy, reversal reaction) to those with progressive infection (lepromatous leprosy).