First Report of Anthracnose on Bletilla striata Caused by Colletotrichum fructicola in China
2021; American Phytopathological Society; Volume: 106; Issue: 2 Linguagem: Inglês
10.1094/pdis-05-21-1008-pdn
ISSN1943-7692
AutoresShaomei Wang, Juan Huang, Miaohua Zheng, Ying-Na Wang, Qing Yuan, Qi Gao, Hao Zhou,
Tópico(s)Mycorrhizal Fungi and Plant Interactions
ResumoHomePlant DiseaseVol. 106, No. 2First Report of Anthracnose on Bletilla striata Caused by Colletotrichum fructicola in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Anthracnose on Bletilla striata Caused by Colletotrichum fructicola in ChinaShaomei Wang, Juan Huang, Miaohua Zheng, Yingna Wang, Qing Yuan, Qi Gao, and Hao ZhouShaomei WangGuangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530006, ChinaSearch for more papers by this author, Juan HuangGuangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530006, ChinaSearch for more papers by this author, Miaohua ZhengGuangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530006, ChinaSearch for more papers by this author, Yingna WangGuangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530006, ChinaSearch for more papers by this author, Qing YuanGuangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530006, ChinaSearch for more papers by this author, Qi Gaohttps://orcid.org/0000-0002-1670-3777Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530006, ChinaSearch for more papers by this author, and Hao Zhou†Corresponding author: H. Zhou; E-mail Address: htmyth@126.comhttps://orcid.org/0000-0001-5909-1902Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530006, ChinaKey Laboratory of Protection and Utilization of Marine Resources, Guangxi University for Nationalities, Nanning 530006, ChinaSearch for more papers by this author AffiliationsAuthors and Affiliations Shaomei Wang1 Juan Huang1 Miaohua Zheng1 Yingna Wang1 Qing Yuan1 Qi Gao1 Hao Zhou1 2 † 1Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530006, China 2Key Laboratory of Protection and Utilization of Marine Resources, Guangxi University for Nationalities, Nanning 530006, China Published Online:10 Jan 2022https://doi.org/10.1094/PDIS-05-21-1008-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleBletilla striata (Thunb.) Rchb. f. (Orchidaceae) is a traditional Chinese medicinal plant. In April 2018 and 2019, a leaf spot disease was observed on ∼20% of B. striata plants in two fields (∼1.4 ha) in Guilin, Guangxi Province, China. Small, circular, brown spots were initially observed on the leaf surfaces, which progressively expanded into large, sunken, dark brown, necrotic areas. As the disease progressed, lesions merged into large, irregular spots, ultimately resulting in abscission. To determine the causal agent, small pieces (5 mm × 5 mm) were collected from the infected leaf tissues (n = 18), surface sterilized in 1% NaOCl for 2 min, and rinsed three times with sterile water. Then, the tissues were placed on potato dextrose agar (PDA) with chloramphenicol (0.1 g/liter) and incubated under a 12-h photoperiod at 26°C for 3 days. Seventeen isolates were obtained, of which 12 isolates with similar morphological characteristics were obtained from the germinated spores on PDA. Seven-day-old colonies on PDA appeared cottony, pale white to pale gray from above, and grayish-green from below. Conidia of strain BJ-101.3 were hyaline, aseptate, straight, and cylindrical, with rounded ends, measuring 11.3 to 15.9 μm × 4.0 to 6.4 μm (n = 50). Appressoria were brown to dark brown, with different shapes and a smooth edge, measuring 6.3 to 10.0 μm × 4.1 to 8.0 μm (n = 50). Morphological features were similar to C. gloeosporioides species complex (Fuentes-Aragón et al. 2018; Weir et al. 2012). For molecular identification, DNA was extracted from two isolates BJ-101.3 and BJ-101.13, following the CTAB method (Guo et al. 2000). The internal transcribed spacer (ITS) region, partial actin (ACT), calmodulin (CAL), chitin synthase (CHS-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), manganese superoxide dismutase (SOD2), beta-tubulin (TUB2), glutamine synthetase (GS), and Apn2-Mat1-2 intergenic spacer and partial mating-type (ApMat) genes were amplified by PCR and sequenced (Silva et al. 2012; Vieira et al. 2017; Weir et al. 2012). The obtained sequences were deposited in GenBank (MW386818, MW386819, MW403508 to MW403519, and MW888410 to MW888413). BLASTn analysis of the obtained sequences showed 99% identity with those of C. fructicola (JX010165, JX010033, FJ917508, FJ907426, JX009866, JX010095, JX010327, JX010405, JQ807838) (Liu et al. 2015; Weir et al. 2012). A phylogenetic tree based on the concatenated sequences confirmed the isolates as C. fructicola. Furthermore, pathogenicity tests were conducted on six 1.5-year-old B. striata plants. Healthy leaves on the plants were inoculated with the conidial suspensions (106 conidia/ml; 10 μl) of the strains BJ-101.3 and BJ-101.13. The conidial suspension of each isolate was inoculated onto at least three leaves. Another three plants inoculated with sterile water served as the control. All plants were covered with transparent plastic bags and incubated in a greenhouse at 26°C for 14 days with a 12-h photoperiod. Nine days postinoculation, the inoculated leaves showed leaf spot symptoms, while the control plants remained symptomless. The experiments repeated three times showed similar results. Finally, C. fructicola was consistently reisolated from the infected leaves and confirmed by morphology and sequencing, fulfilling Koch’s postulates. The outcome of this study will help in developing effective management measures against anthracnose of B. striata.The author(s) declare no conflict of interest.References:Fuentes-Aragón, D., et al. 2018. 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Huang contributed equally to this manuscript.Funding: This work was supported by the Guangxi University for Nationalities Research Funding Project (grant no. 2018KJQD16) and the National Natural Science Foundation of China (grant no. 32060599).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 2 February 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionWater-soaked lesions on bougainvillea caused by Robbsia andropogonis (O. Morales-Galván et al.). Photo credit: L. F. Flores-López. Soybean leaf showing crinkling and downward curling, characteristic of infection by soybean mosaic virus (SMV) (S. van Bentum et al.). Photo credit: S. van Bentum. Metrics Article History Issue Date: 28 Feb 2022Published: 10 Jan 2022First Look: 2 Jul 2021Accepted: 30 Jun 2021 Page: 756 Information© 2022 The American Phytopathological SocietyFundingGuangxi University for Nationalities Research Funding ProjectGrant/Award Number: 2018KJQD16National Natural Science Foundation of ChinaGrant/Award Number: 32060599Keywordspathogen detectionfungiherbaceous/flowering plantsThe author(s) declare no conflict of interest.PDF download
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