First Report of Colletotrichum fructicola Causing Anthracnose on Cherry ( Prunus avium ) in China
2021; American Phytopathological Society; Volume: 106; Issue: 1 Linguagem: Inglês
10.1094/pdis-03-21-0544-pdn
ISSN1943-7692
AutoresZhaoyang Tang, Jun Lou, Luqian He, Qidong Wang, Linghui Chen, Xueting Zhong, Choufei Wu, Liqin Zhang, Zhanqi Wang,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 106, No. 1First Report of Colletotrichum fructicola Causing Anthracnose on Cherry (Prunus avium) in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Colletotrichum fructicola Causing Anthracnose on Cherry (Prunus avium) in ChinaZhaoyang Tang, Jun Lou, Luqian He, Qidong Wang, Linghui Chen, Xueting Zhong, Choufei Wu, Liqin Zhang, and Zhan Qi WangZhaoyang TangKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, 313000, China, Jun LouKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, 313000, China, Luqian HeCollege of Life Sciences, Huzhou University, Huzhou, 313000, China, Qidong WangCollege of Life Sciences, Huzhou University, Huzhou, 313000, China, Linghui ChenTaizhou Academy of Agriculture Sciences, Taizhou, 317000, China, Xueting ZhongKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, 313000, China, Choufei WuKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, 313000, China, Liqin Zhang†Corresponding authors: L. Zhang; E-mail Address: zhangliqin@zjhu.edu.cn, and Z. Q. Wang; E-mail Address: zhqwang@zju.edu.cnKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, 313000, China, and Zhan Qi Wang†Corresponding authors: L. Zhang; E-mail Address: zhangliqin@zjhu.edu.cn, and Z. Q. Wang; E-mail Address: zhqwang@zju.edu.cnhttps://orcid.org/0000-0002-7953-6128Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, 313000, China AffiliationsAuthors and Affiliations Zhaoyang Tang1 Jun Lou1 Luqian He2 Qidong Wang2 Linghui Chen3 Xueting Zhong1 Choufei Wu1 Liqin Zhang1 † Zhan Qi Wang1 † 1Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, 313000, China 2College of Life Sciences, Huzhou University, Huzhou, 313000, China 3Taizhou Academy of Agriculture Sciences, Taizhou, 317000, China Published Online:29 Dec 2021https://doi.org/10.1094/PDIS-03-21-0544-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleCherry (Prunus avium) is an economically important fruit in China. In October 2020, a leaf spot disease was found on cherry in the orchard of Taizhou Academy of Agriculture Sciences, Zhejiang, China. Symptoms appeared as small, water-soaked spots on the leaves, which later became larger, dark brown, and necrotic lesions of 1 to 3 × 4 to 8 cm. Disease incidences of ∼60% of leaves were observed by sampling five locations. To isolate the causal agent, small fragments from five target symptomatic leaves were surface sterilized with 1.0% NaOCl solution for 1 min and then rinsed three times with sterilized water. Then the leaf fragments were air dried, plated on PDA, and incubated at 25°C in the dark for 2 days. Pure cultures were obtained by transferring hyphal plug of 2 mm diameter onto PDA, which followed single-spore isolation. Colony morphology showed light to dark gray, cottony mycelium, and the underside of the culture became brownish after 7 days. Conidia (n = 28) were hyaline, smooth-walled, cylindrical, aseptate, broadly rounded ends, and average size around 3.84 × 12.82 μm (2.99 to 4.87 × 10.27 to 15.68 μm). Appressoria (n = 27) were mostly brown, ovoid, and slightly irregular in shape, with average size around 8.04 × 9.68 μm (6.29 to 9.67 × 9.32 to 12.06 μm). Perithecia average size is 106.25 μm, textura angularis, thick-walled. Asci 26.35 to 49.18 × 5.00 to 12.03 μm (average size 37.44 × 7.80 μm, n = 17), unitunicate, thin-walled, clavate or cymbiform. Ascospores 13.69 to 20.93 × 3.86 to 6.69 μm (average size 16.00 × 5.42 μm, n = 30), one-celled, hyaline, one or two large guttulate at the center, slightly rounded ends. Morphological characteristics matched well with previous descriptions of Colletotrichum species of C. gloeosporioides species complex, including Colletotrichum fructicola (Fu et al. 2019; Prihastuti et al. 2009). The identity of two representative isolates (cf2-3 and cf4-4) from different leaves was confirmed by multilocus gene sequencing. To this end, genomic DNA was extracted by the Plant Direct PCR kit (Vazyme Biotech, China). Molecular identification was conducted by sequencing the internal transcribed spacer (ITS) rDNA region, partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, partial actin (ACT) gene, partial beta-tubulin 2 gene (TUB2), and partial chitin synthase gene (CHS). The obtained sequences were deposited in GenBank under accession numbers MW581851 and MW581852 (ITS), MW590586 and MW590587 (GAPDH), MW616561 and MW616562 (ACT), MW729380 and MW729381 (TUB2), and MW729378 and MW729379 (CHS). BLAST analysis revealed that the ITS, GAPDH, ACT, TUB2, and CHS sequences of both isolates matched with 100% identity to C. fructicola culture collection sequences in GenBank database (JX010165, JX009998, JX009491, JX010405, and JX009866, respectively). These morphological characteristics and molecular analyses allowed the identification of the pathogen as C. fructicola. Koch's postulates were performed with healthy detached cherry leaves of cultivar 'HongMi' from Taizhou Academy of Agriculture Sciences. Surface-sterilized leaves were inoculated with 5-day-old cultures of C. fructicola mycelial discs of 2 mm diameter after being wounded with a needle or nonwounded. Control leaves were inoculated with same size discs of PDA agar. Treated leaves were incubated at 25°C in the dark at high relative humidity. Anthracnose symptoms appeared within 3 days both on nonwounded and wounded inoculation approaches. Mock-inoculated controls remained asymptomatic. Biological repetitions were carried out three times. The fungus was reisolated from infected leaves and confirmed as C. fructicola following the methods described above. Until recently, it has been found that C. fructicola can infect tea, apple, pear, and Pouteria campechiana in China (Fu et al. 2013; Li et al. 2013; Shi et al. 2018; Yang et al. 2021). To the best of our knowledge, this is the first report of C. fructicola on cherry in China.The author(s) declare no conflict of interest.References:Fu, D., et al. 2013. Mycotaxon 126:23. https://doi.org/10.5248/126.23 Crossref, ISI, Google ScholarFu, M., et al. 2019. Persoonia 42:1. Crossref, ISI, Google ScholarLi, H., et al. 2013. Plant Dis. 97:1000. https://doi.org/10.1094/PDIS-01-13-0084-PDN Link, ISI, Google ScholarPrihastuti, H., et al. 2009. Fungal Divers. 39:89. ISI, Google ScholarShi, N., et al. 2018. Plant Dis. 102:241. https://doi.org/10.1094/PDIS-05-17-0705-PDN Link, ISI, Google ScholarYang, Z., et al. 2021. Plant Dis. 105:708. https://doi.org/10.1094/PDIS-02-20-0253-PDN Link, ISI, Google ScholarZ. Tang and J. Lou contributed equally to this work.Funding: This work was supported by the Zhejiang Key R&D Program of China (2019C02030) and the Zhejiang Basic Public Welfare Research Project (LGN19C140002).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 1 January 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms of Macrophomina phaseolina in melon (R. Cohen et al.). Photo credit: R. Cohen. Jute plant infected with papaya ring spot virus (PRSV) (sample MG16-004) (C. Biswas et al.). Photo credit: V. Ramesh Babu. Metrics Article History Issue Date: 7 Feb 2022Published: 29 Dec 2021First Look: 16 Jul 2021Accepted: 14 Jul 2021 Page: 317 Information© 2021 The American Phytopathological SocietyFundingZhejiang Key R&D Program of ChinaGrant/Award Number: 2019C02030Zhejiang Basic Public Welfare Research ProjectGrant/Award Number: LGN19C140002KeywordsColletotrichum fructicolacherryanthracnoseThe author(s) declare no conflict of interest.PDF download
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