Portal de Periódicos da CAPES

Efecto antibacteriano del extracto etanólico de Annona muricata sobre microorganismos de importancia clínica

2021; Volume: 44; Issue: 1 Linguagem: Espanhol

2227-3662

Cinthya Lizet Castro Hernández, José Gabriel Ayasta Senmache, Cinthya Yanina Santa Cruz-López, Fransk Amarildo Carrasco Solano, Mario Moreno-Mantilla,

Traditional and Medicinal Uses of Annonaceae

espanolEl incremento de cepas patogenas resistentes a farmacos convencionales ha limitado las opciones de tratamiento medico. Ante ello surge la necesidad de buscar alternativas terapeuticas. Muchas especies vegetales poseen un enorme potencial antimicrobiano que puede ser de gran utilidad. Objetivo: determinar el efecto antibacteriano in vitro del extracto etanolico de Annona muricata L. sobre Staphylococcus aureus, Streptococcus B – hemoliticos y Escherichia coli. Metodos: se evaluaron 135 unidades experimentales conformadas por 3 cepas de Staphylococcus aureus, Streptococcus B-hemoliticos y Escherichia coli, ademas de 5 concentraciones del extracto y 3 repeticiones del experimento. Para determinar el efecto antibacteriano in vitro se emplearon los metodos de disco difusion en agar y macrodilucion en caldo. Se utilizo el extracto etanolico a concentraciones de 125, 250, 500, 750, 1000 mg/ml y solucion salina fisiologica esteril como control negativo. Resultados: el extracto inhibio el crecimiento in vitro de Staphylococcus aureus y Streptococcus B-hemoliticos. La mayor inhibicion se observo a 1 000 mg/ml con halos inhibitorios de 14,6 mm y 12,33 mm de diametro, respectivamente. Para Escherichia coli no se observo la formacion de halos inhibitorios. Las cepas de Streptococcus B-hemoliticos y Staphylococcus aureus presentaron una concentracion minima inhibitoria de 250 y 500 mg/ml, respectivamente. Conclusion: el efecto antibacteriano in vitro fue directamente proporcional a cada concentracion empleada sobre Staphylococcus aureus y Streptococcus B-hemoliticos. En el caso Escherichia coli no se observo inhibicion de crecimiento. EnglishThe increase in pathogenic strains resistant to conventional drugs has limited medical treatment options. Given this, the need to seek therapeutic alternatives arises. Many plant species have enormous antimicrobial potential that can be very useful. Objective: to determine in vitro the antibacterial effect of the ethanolic extract of Annona muricata L. on Staphylococcus aureus, Streptococcus B - hemolytic and Escherichia coli. Methods: 135 experimental units were evaluated, consisting of 3 strains of Staphylococcus aureus, Streptococcus B-hemolytic and Escherichia coli, in addition to 5 concentrations of the extract and 3 repetitions of the experiment. To determine the antibacterial effect in vitro, the agar diffusion disk and broth macrodilution methods were used. The ethanolic extract was used at concentrations of 125, 250, 500, 750, 1000 mg / ml and sterile physiological saline solution as negative control. Results: the extract inhibited in vitro growth of Staphylococcus aureus and Streptococcus B-hemolytic. The greatest inhibition was observed at 1000 mg / ml with inhibitory halos of 14.6 mm and 12.33 mm in diameter, respectively. For Escherichia coli the formation of inhibitory halos was not observed. The Streptococcus B-hemolytic and Staphylococcus aureus strains presented a minimum inhibitory concentration of 250 and 500 mg / ml, respectively. Conclusion: the in vitro antibacterial effect was directly proportional to each concentration used on the Staphylococcus aureus and Streptococcus B-hemolytic. In the case of Escherichia coli, no growth inhibition was observed.