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Effect of time in culture on fatty acid composition of copepods Tisbe sp . and Apocyclops sp .

2021; Wiley; Volume: 53; Issue: 2 Linguagem: Inglês

10.1111/jwas.12827

1749-7345

Ruth Milagros Alejos-Cabrera, Wilmer A. Gaspar Reyes, Leenin Flores Ramos, Gheraldine A. Ynga Huamán, Anthony Ruíz Soto, Alexander F. Niño Velásquez,

Marine Bivalve and Aquaculture Studies

Abstract The purpose of this study was to evaluate the effect of time in culture on fatty acid composition of copepods Tisbe sp . and Apocyclops sp . For experiments, Tisbe and Apocyclops copepodites were separated and placed in 4‐L containers at 5 ind/mL. The fatty acids were evaluated at three different periods (hours) of culture (treatments): T1 = 6 hr, T2 = 12 hr, and T3 = 168 hr. Culture conditions were 25°C, salinity 35 ppt, illuminance 2,500 lx, with gentle aeration. The live food was Isochrysis galbana at 75 × 10 3 cells/mL. A total of 14 fatty acids were detected for both copepods. Although, there were absent two fatty acids for Apocyclops sp . (C18:3n‐6 and C18:1n‐7). The major percentages of fatty acids were more than 50% of polyunsaturated fatty acids. The essential fatty acids were detected such as C22:6n‐3 (docosahexaenoic acid [DHA]), C20:5n‐3 (eicosapentaenoic acid [EPA]), and C20:4n‐6 (arachidonic acid) for both species. Tisbe sp . was the species which shows the highest quantity of total lipids compared to Apocyclops sp . for the three treatments. Besides, in T3 both species had greater quantities of total lipids in respect to the first treatment. The ratio of DHA/EPA was optimal for culture of fish marine larvae because the proportion is higher than 2:1.