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Apoptosis in the late replication phase of Bovine alphaherpesvirus 1 in experimentally infected calves

2021; Springer Nature; Volume: 52; Issue: 4 Linguagem: Inglês

10.1007/s42770-021-00546-8

1678-4405

Hanna Carolina Campos Ferreira, Elaine Nery Araujo, Nívia Carolina Lopes Rosado, Juliana Lopes Rangel Fietto, Marcus Rebouças Santos, Lidiany Lopes Gomes, Laura Morais Nascimento Silva, Gustavo Costa Bressan, Gustavo Ferreira Martins, Srinand Sreevatsan, Abelardo Silva‐Júnior,

Toxoplasma gondii Research Studies

Bovine alphaherpesvirus 1 (BoHV-1) is a pathogen causing respiratory and reproductive clinical signs in cattle. Infected animals may develop rhinotracheitis, vulvovaginitis, balanoposthitis, and abortion. Viral latency is generally established in neuronal ganglia simultaneously to a decrease in both genes or genome expression and viral replication. Under stressful conditions, infection is reactivated leading to viral replication and the manifestation of clinical signs. In this study, we evaluated both viral reactivation and apoptosis in trigeminal ganglia cells as BoHV-1 progressed from the latent to the acute phase of infection after dexamethasone administration in experimentally infected calves. To test ganglia cell death as a consequence of BoHV-1 infection, we stained the BoHV-1 samples with TUNEL after the viral shedding by the calves. RT-qPCR of apoptotic genes was also performed, showing the upregulation of the caspase 8 gene in the trigeminal ganglia from cattle experimentally infected with BoHV-1. These results showed the occurrence of apoptosis in ganglion cells of calves infected by BoHV-1.