Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Phenolic-rich apple extracts have photoprotective and anti-cancer effect in dermal cells

2021; Elsevier BV; Volume: 1; Issue: 4 Linguagem: Inglês

10.1016/j.phyplu.2021.100112

2667-0313

Chirle de Oliveira Raphaelli, Jéssica Gonçalves Azevedo, Elisa dos Santos Pereira, Juliana Vinholes, Taiane Mota Camargo, Jéssica Fernanda Hoffmann, Jardel Araújo Ribeiro, M. Vizzotto, César Valmor Rombaldi, Michaël Wink, Elizandra Braganhol, Leonardo Nora,

Skin Protection and Aging

Skin cancer is the most common type of malignancy in light-skinned populations and phenolics are promising anticarcinogenic agents. To characterise and evaluate the protective potential of apple extracts against the DNA damage caused by UV-radiation in culture of human fibroblasts as well as to verify the anticarcinogenic effect of these extracts in murine and human melanoma cells. Acetone-ethanol extracts of apple were purified and fractionated by solid phase extraction. Four phenolic fractions (PF-I, PF-II, PF-III, PF-IV) and one Unfractionated Phenolics (UFP) were obtained. The presence of amygdalin, hydroxycinnamic acid, flavonols, flavanols and dihydrochalcones was confirmed by High-Performance Liquid Chromatography–Mass Spectrometry. Following 48 h exposure to UV-radiation, UFP, PF-III and PF-IV protected (14–34 %) fibroblast DNA against UV-radiation; the UFP and PF-IV, both at 31.25 μg.mL−1, showed high antiproliferative effect in murine melanoma cells (B16F10); UFP and PF-IV, both at 125 μg.mL−1, and PF-II, at 250 μg.mL−1, were effective against human melanoma cells (SK-Mel-103). The results indicate that phenolic extracts from apple (Malus domestica Borkh cv. Gala) have potential for use in the formulation of cosmetic products and/or medicines for the protection of cellular DNA against UV radiation and for the treatment of melanoma.